Protein ligands were conjugated to the surface of biodegradable microspheres. These microsphere-ligand conjugates were then used in two in vitro model systems to evaluate the effect of conjugated ligands on microsphere behavior. Microsphere retention in agarose columns was increased by ligands on the microsphere surface specific for receptors on the agarose matrix. In another experiment, conjug...

Background: The ethno-medical significance of Clerodendrum genus raises the interest towards the characterization of its seed lectin by inexpensive and most effective technique.Objective: The focus of this study is the purification, characterization, and evaluation of the antioxidant and antiproliferative potential of a galactose-specific lectin from Cler...

Global Krylov subspace methods are the most efficient and robust methods to solve generalized coupled Sylvester matrix equation. In this paper, we propose the nested splitting conjugate gradient process for solving this equation. This method has inner and outer iterations, which employs the generalized conjugate gradient method as an inner iteration to approximate each outer iterate, while each...

aflatoxins are a group of fungal toxic metabolites, which are contaminated certain food commodities. elisa is one of the sensitive methods for detection of aflatoxins. preparation and stabilizing of a proper conjugated tracer for detection of aflatoxins is probably the main step for designing an elisa method. in current study, different stabilizers were applied to stabilize a newly prepared con...

Membrane fractions from seedlings of four soybean [Glycine max (L.) Merr.] lines were examined by radioimmunoassay and hemagglutination assay for the 120,000 dalton soybean lectin. Two of the lines (Sooty and T-102) are genotypically lele and lack buffer-soluble soybean lectin; the remaining two lines (Beeson and Harosoy 63) are Le and produce seeds that contain the lectin (Su et al. 1980 Bioch...

Many fungi are known to secrete lectins, but their functional roles are not clearly understood. Sclerotium rolfsii, a soilborne plant pathogenic fungus capable of forming fruiting bodies called sclerotial bodies, secrete a cell wall-associated Thomsen-Friedenreich antigen-specific lectin. To understand the functional role of this lectin, we examined its occurrence and expression during developm...

An endogenous beta-galactoside-specific lectin has previously been isolated from rabbit bone marrow. The quantification of extracted lectin now indicates that approximately 75% of the lectin is intracellular in marrow. Indirect immunofluorescence studies show the extracellular lectin is associated with the erythroblast cell surface and is also found in some acellular areas of the marrow stroma....

The isolation of lectins by affinity chromatography with crosslinked hemicelluloses has been a common practice because the variety glycosides that they present, improving different kinds lectins, such as galactose ligands.Lectins for carbohydrates is so specific simple configuration chiral carbon can affect affinity, and there are more related to alfa-galactosidic than beta-galactosidic residue...

The purpose of this article is to report that some anti-A1 react at 37°C are IgG antibodies and clinically significant, as they can cause the destruction a proportion A1 cells in vivo following transfusion red blood cells. Therefore, when serum an individual contains anti-A1, further testing plasma against group A1, A2, O by referencing lectin (Dolichos biflorus lectin) must be performed. Confi...