We have combined a confocal laser scanning head modified for TPE (two-photon excitation) microscopy with some spectroscopic modules to study single molecules and molecular aggregates. The behavior of the TPE microscope unit has been characterized by means of point spread function measurements and of the demonstration of its micropatterning abilities. One-photon and two-photon mode can be simply...

This study was performed to confirm that activated leukocytes are concerned with Vascular Shut Down effect ; VSD in PhotoDynamic Therapy ; PDT. Hydrogen peroxide ;H2O2, which is a kind of active oxygen that came from monocytes, was made visibility under the confocal laser scanning microscope ;CLSM, and tried to quantify the amount of its formation from the fluorescent intensity with image analy...

A krypton-fluorine excimer laser at a 248-nm wavelength was used to irradiate normal and severely atherosclerotic segments of human postmortem femoral arteries. Single pulses and multiple pulses required for penetration or perforation of the arterial wall were applied with 16 nsec pulse width and 5 J/cm2/pulse energy fluence. The total fluorescence of irradiated and ablated tissue was analyzed ...

The temperature dependence of the fluorescence emission of certain organic dyes is utilized for measuring the temperature in liquid flows. Measurements are generally based on the assumptions that: the fluorescence signal is proportional to the intensity of the laser excitation, and the temperature sensitivity of the dye is not affected by the laser irradiance. These assumptions allow justifying...

Complexation of metallic silver nano particles (NPs) with Pyrromethene laser dyes (PM567, PM597) has significant effect on lifetime, photostability and the fluorescence efficiency. These enhancement of optical properties of the complex in solution due to surface Plasmon resonance (SPR) have been promised higher efficiencies in case of [40% Ag NPs: PM dye] complexes for dye laser systems. The fl...

A two-wavelength laser-induced fluorescence technique is described for detecting and classifying biological aerosols. Single aerosols, smaller than 10 mm, are interrogated with 266 nm and 355 nm laser pulses separated in time by 400 ns. Fluorescence signals excited by these pulses are detected in three broad spectral bands centered at 350 nm, 450 nm and 550 nm. The results indicate that bacteri...

We show that far-field fluorescence nanoscopy by stimulated emission depletion (STED) can be realized with compact off-the-shelf laser diodes, such as those used in laser pointers and DVDs. A spatial resolution of 40-50 nm is attained by pulsing a 660 nm DVD-diode. The efficacy of these low-cost STED microscopes in biological imaging is demonstrated by differentiating between clusters of the sy...

A new lead fluoroborate glass (PbO-PbF2-B2O3) doped with ytterbium (Yb:PbFB) is presented. Samples with different concentrations of Yb3+ were produced and had their emission cross-sections, fluorescence lifetimes and minimum pump intensities determined. They have high refractive index of 2.2 and a density of 4.4 g/cm3. For a doping level of 1.153x1020 ions/cm3, the fluorescence lifetime, after ...

We present an optical trapping system combining individually addressable multiple laser traps with fluorescence spectroscopy. An in-line set of 64 near-IR laser diodes is used to create a line of individually addressable traps inside a microfluidic chip. This system is completed by an excitation/detection line for spectrally resolved fluorescence imaging of trapped particles. Highly parallel tr...

A means of performing simultaneous, high-speed measurements of temperature and OH lifetime-corrected laser-induced fluorescence (LIF) for tracking unsteady flames has been developed and demonstrated. The system uses the frequency-doubled and frequency-tripled output beams of an 80 MHz mode-locked Ti:sapphire laser to achieve ultrashort laser pulses (order 2 ps) for Rayleigh-scattering thermomet...