Cloning of DNA fragments in phage M13 is a routine procedure in DNA sequence determination. A simple procedure for the determination of DNA fragment orientation has been described earlier (1). Here we introduce a modification of this procedure which allows the simultaneous determination of DNA fragment orientation and size. Procedure: Single-stranded M13 DNAs (1, about lpg) were mixed in 5ul of...

-In this paper, a Catmull-Rom spline based lane model which described the perspective effect of parallel lines was proposed for generic lane boundary. As Catmull-Rom spline can form arbitrary shapes by control points, it can describe a wider range of lane structures than other lane models such as straight and parabolic model. It formulates the lane detection problem in the form of determining t...

This paper addresses the problem of the navigation of a mobile vehicle in outdoor environment. A CatmullRom Spline based lane model which described the perspective effect of parallel lines was proposed for generic lane boundary. As Catmull-Rom Spline can form arbitrary shapes by control points, it can describe a wider range of lane structures than other lane models such as straight and paraboli...

Modern cars are incorporating an increasing number of driver assist features, among which automatic lane keeping. The latter allows the car to properly position itself within the road lanes, which is also crucial for any subsequent lane departure or trajectory planning decision in fully autonomous cars. Traditional lane detection methods rely on a combination of highly-specialized, hand-crafted...

Automatic response to lane-blocking incidents is a critical issue in the field of automated highway systems (AHS). Accordingly, this paper presents a microscopic vehicular control methodology for automatic-control (AC) vehicular movements in response to lane-blocking incidents in the AHS environment. The embedded traffic control logic is based on the basic safety requirements for automatic-cont...

The objectives of this manuscript are (1) to evaluate the effectiveness of on-street bicycle lane in reducing crashes involving bicyclists on urban roads, (2) to quantify and compare risk to bicyclists on road segments with and without on-street bicycle lane, (3) to evaluate the effect of on-street bicycle lane on other road network users (all crashes), and, (4) to assess the role of on-network...

Objective: We investigated the impacts of drivers’ socio-demographic factors on these lane-changing models, and developed Fuzzy logic-based lane-changing models associated the drivers’ demographic factors. Methods: Forty drivers were recruited for a driving simulator test to collect their driving behaviors of changing lane in a work zone with/without the aid of Driver’s Smart Advisory System (D...

|This paper will report new results we have obtained in applying stereo vision algorithms to the problem of autonomous vehicle navigation on highways. The project consists of two parts: lane extraction and obstacle detection. Our lane extraction system is based on a parameterized model for the appearance of the lanes in the images. This model captures the position, orientation and width of the ...

In this paper we explore the time course of a lane change in terms of the driver’s control and eyemovement behavior. We conducted an experiment in which drivers navigated a simulated multi-lane highway environment in a fixed-base, medium-fidelity driving simulator. We then segmented the driver data into standardized units of time to facilitate an analysis of behavior before, during, and after a...