analysis of the Lactococcus lactis dnaJ gene.

Lactococcus lactis subsp. lactis strains show glutamate decarboxylase activity, whereas L. lactis subsp. cremoris strains do not. The gadB gene encoding glutamate decarboxylase was detected in the L. lactis subsp. cremoris genome but was poorly expressed. Sequence analysis showed that the gene is inactivated by the frameshift mutation and encoded in a nonfunctional protein.

1. Murabito E, Verma M, Bekker M, Bellomo D, Westerhoff HV, et al. (2014) Monte-Carlo Modeling of the Central Carbon Metabolism of Lactococcus lactis: Insights into Metabolic Regulation. PLoS ONE 9(9): e106453. doi:10.1371/ journal.pone.0106453 Citation: The PLOS ONE Staff (2014) Correction: Monte-Carlo Modeling of the Central Carbon Metabolism of Lactococcus lactis: Insights into Metabolic Reg...

Reduced genome strain for threonine production http://www.microbialcellfactories.com/content/8/1/2 This paper deals with a key concept in genome engineering; specifically, genome reduction to remove unneeded functions and to enhance desirable cell phenotypes for biotechnology. Genomic engineering Lactococcus lactis http://www.nisr.or.jp/englishHP/report2007/ NISR07hashimoto.pdf Genome deletion ...

INTRODUCTION A case of Lactococcus lactis cremoris causing cholangitis is described. This Gram-positive organism is not routinely considered to be pathogenic in immunocompetent individuals. To our knowledge, this is the thirteenth report of invasive infection and the first of cholangitis to be reported in association with this organism. CASE PRESENTATION A 72-year-old patient presented with C...

Lactococcus lactis subsp. lactis biovar. diacetylactis DPC721 is a spontaneous bacteriophage insensitive mutant of strain DPC220, isolated after challenge with an industrial bacteriophage, phi D1. Plasmid analysis demonstrated that the bacteriophage insensitivity was associated with the absence of two native DPC220 plasmids (pAH82 and pAH33), and the presence of a novel plasmid (pAH90) in DPC72...

Potato starch waste, a chips industry effluent, was used for the production of industrially important enzymes by an amylolytic strain of lactic acid bacteria, isolated from pickled yam & identified as Lactococcus lactis. The strain was observed to co-produce α-amylase and β-galactosidase. Potato starch waste was efficiently utilized (91.6%) along with high co-production of α-amylase and β-galac...

Hydroxyacid dehydrogenases limit the conversion of α-keto acids into aroma compounds. Here we report that inactivation of the panE gene, encoding the α-hydroxyacid dehydrogenase activity in Lactococcus lactis, enhanced the formation of 3-methylbutanal and 3-methylbutanol. L. lactis IFPL953ΔpanE was an efficient strain producing volatile compounds related to cheese aroma.

Lactococcus lactis is a lactic acid bacterium used in the production of many fermented foods, such as dairy products. Here, we report the genome sequence of L. lactis subsp. lactis TOMSC161, isolated from nonscalded curd pressed cheese. This genome sequence provides information in relation to dairy environment adaptation.

Lactococcin, a bacteriocin produced by Lactococcus lactis subsp. lactis ADRIA 85LO30, was purified as a 2.3-2.4 kDa peptide. Six non-bacteriocin-producing (Bac-) and non-immune (Imm-) strains were isolated after curing experiments. These strains had in common the loss or modification of two plasmids: pOS4 (32 kb) and pOS5 (70 kb). By comparing pOS5 and several modified plasmids, a DNA region fr...