Lactic acid, commonly used in food, chemical and pharmaceutical industries, has recently received much attention for the production of biodegradable plastics. In this study, Rhizopus oryzae MTCC 8784 was used to convert starch into optically pure L-Lactic acid. Effect of time, pH, addition of calcium carbonate and starch concentration were analyzed using shaker flasks in order optimize the L-la...

Growth of lactic acid bacteria and amino acid production at an Indonesian soy sauce manufacturer, employing modern Japanese process technology, indicated that brine fermentation for one month is sufficient for industrial kecap production. Compared to traditional Indonesian soy sauce fermentation, application of modern Japanese process technology resulted in an obvious but not essential yeast fe...

In vitro fermentation experiments and a feeding trial were conducted to determine how distillers byproducts impact rumen microbiology and metabolism. The in vitro rate of lactic acid disappearance was not stimulated by direct addition of distillers byproducts to mixed rumen contents collected from a steer adapted to a high concentrate diet. However, if animals were fed condensed distillers bypr...

A global model of the lactic fermentation step of gowé was developed by assembling blocks hosting models for bacterial growth, lactic acid production, and the drop of pH during fermentation. Commercial strains of Lactobacillus brevis and of Lactobacillus plantarum were used; their growth was modeled using Rosso's primary model and the gamma concept as a secondary model. The optimum values of pH...

We developed a new cell surface engineering system based on the PgsA anchor protein from Bacillus subtilis. In this system, the N terminus of the target protein was fused to the PgsA protein and the resulting fusion protein was expressed on the cell surface. Using this new system, we constructed a novel starch-degrading strain of Lactobacillus casei by genetically displaying alpha-amylase from ...

In this work, autochthonous lactic acid bacteria (LAB) were isolated from chia (Salvia hispanica L.) dough and selected on the basis of the kinetics of acidification and proteolytic activity. Strain no. C8, identified as Lactobacillus plantarum C8, was selected and used as starter to obtain chia sourdough. Lactic acid fermentation increased the organic acid mass fractions (lactic, acetic and ph...

Novel solid supports, consisting of polypropylene blended with various agricultural materials (pp composite), were evaluated as supports for pure- and mixed-culture continuous lactic acid fermentations in biofilm reactors. Streptomyces viridosporus T7A (ATCC 39115) was used to form a biofilm, and Lactobacillus casei subsp. rhamnosus (ATCC 11443) was used for lactic acid production. For mixed-cu...

Lactobacillus casei, a homofermentative lactic acid bacterium, was shown by Gibbs et al. (1950) to ferment glucose-i-C4 to products labeled as predicted for the classical EmbdenMeyerhof glycolytic scheme, whereas Leuconostoc mesenteroides strain 39, a heterofermentative lactic acid coccus, gave product labeling incompatible with an Embden-Meyerhof pathway and was interpreted as indicative of th...

Fermented buckwheat sprouts (FBS) are used as multifunctional foods. Their production process includes fermentation with lactic acid bacteria. The major strains were found to include Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus pentosus, Lactococcus lactis subsp. lactis, and Pediococcus pentosaceus in an investigation of the lactic acid bacteria. We searched for the functional c...

Batch fermentation of milk inoculated with lactic acid bacteria was conducted in the presence of hydrotalcite-type anionic clay under static and ultrasonic conditions. An experimental study of the effect of fermentation temperature (t=38-43 °C), clay/milk ratio (R=1-7.5 g/L) and ultrasonic field (ν=0 and 35 kHz) on process dynamics was performed. A mathematical model was selected to describe th...