Transport of glucose across the plasma membrane of mammary epithelial cells is believed to be a passive process of facilitated diffusion mediated by facilitative glucose transporter(s). This article presents three lines of evidence that indicate the expression of sodium/glucose cotransporter (SGLT1) in the mammary gland of lactating and nonlactating cows. First, transcripts of SGLT1 mRNA rangin...

An electronic probe with parallel stainless steel electrodes was designed to measure changes in electrical resistance in the anterior vagina associated with increased cervical mucus secretion at estrus. Three groups of Holsteins were probed, and a sample of residual milk after milking was taken for progesterone assay every 2nd day for 28 to 40 days. Group I consisted of 29 cycling virgin heifer...

Two experiments studied the effects of canola or sunflower seed supplementation on conjugated linoleic acid (CLA) concentration in milk fat of lactating cows. In each experiment, only eight cows in early lactation were available to assign to one of two treatments in a completely randomized design and were fed individually for 12 wk. Experiment 1 compared similar diets with or without the additi...

Two experiments were conducted to evaluate an accelerometer system (Heatime; SCR Engineers Ltd., Netanya, Israel) to manage reproduction in lactating dairy cows. In experiment 1, lactating Holstein cows (n=112) were fitted with an accelerometer system and were treated with GnRH followed 7d later by PGF(2α) to synchronize estrus. A total of 89 cows that had a follicle >10mm in diameter and a fun...

in this investigation 13699 data related to milk production from 2716 holstein first calving and daughter from 167 sire cows were analyzed based on random regression model (rr/cf) and reml method incomplete lactating (5-90 days) and (5-120 days) in correlations to complete lactating were 0.67 and 0.72, respectively with progress in complete 5-240 day was 0.91 the correlation value for lactating...

BACKGROUND Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne's disease (JD), or paratuberculosis in ruminants has been suspected to be implicated in the pathogenesis of Crohn's disease (CD) in humans with chronic inflammatory intestinal changes. As the hypothesis is now fast being recognized that MAP could possibly be the etiological agent of CD which is foun...

BACKGROUND Results regarding the use of bovine somatotropin for enhancing fertility in dairy cattle are variable. Here, the hypothesis was tested that a single injection of a sustained-release preparation of bovine somatotropin (bST) during the preovulatory period would improve pregnancy success of lactating dairy cows at first service. RESULTS The first experiment was conducted in a temperat...

The objective of this study was to determine the effect of GnRH (100 microg i.m.) treatment 5 and 15 days after timed insemination (TAI) on pregnancy rate and pregnancy loss in lactating dairy cows subjected to synchronization of ovulation. The study included 831 lactating dairy cows subjected to a Presynch-Ovsynch protocol for first service. On the day of TAI (Day 0), cows were randomly assign...

Three experiments were conducted to investigate the effects of polyurethane coated urea on in vitro ruminal fermentation, ammonia release dynamics and lactating performance of Holstein dairy cows fed a steam-flaked corn-based diet. In Exp. 1, a dual-flow continuous culture was run to investigate the effect of polyurethane coated urea on nutrient digestibility, rumen fermentation parameters and ...

An adequate and clean artificial insemination (AI) technique is recommended to improve reproductive outcomes in dairy cattle. The objective of this study was to evaluate the effectiveness of using protective plastic sheaths (PS) to minimize contamination of the AI catheter (AIC) on pregnancies per AI (PAI) in lactating dairy cattle. Lactating cows housed in freestall barns on a commercial dairy...