Some selected amino acids, in particular L-aspartic acid (L-Asp) and L-histidine (L-His), can function as leaving group during polymerase-catalyzed incorporation of deoxyadenosine monophosphate (dAMP) in DNA. Although L-Asp-dAMP and L-His-dAMP bind, most probably, in a different way in the active site of the enzyme, aspartic acid and histidine can be considered as mimics of the pyrophosphate mo...

In a continuation of work on gene-enzyme relationships in histidine biosynthesis (1, 2) we have examined the early steps of the biosynthetic pathway in Salmonella typhimurium. It has been found that the first enzyme of the pathway catalyzes the condensation of 5-phosphoribosyl-1-pyrophosphate and adenosine triphosphate to form pyrophosphate and a product which has been isolated and characterize...

Amino acid ionic liquid-supported Schiff bases, derivatives of salicylaldehyde and various amino acids (L-threonine, L-valine, L-leucine, L-isoleucine and L-histidine) have been investigated by means of various spectroscopic techniques (NMR, UV-Vis, IR, MS) and deuterium isotope effects on ¹³C-NMR chemical shifts. The results have shown that in all studied amino acid ionic liquid-supported Schi...

At pH values where it is predominantly without net charge, histidine in dilute solution divided its uptake by the Ehrlich cell between the Na+-independent L system and the Na+-requiring A system. As in every other case studied the L component was partially inhibitable by lysine, partially not. At pH 5, where it is chiefly a cation, an additional component became perceptible, one which could be ...

To simulate the types of coordination and solution structures of the active site of haloperoxidases, the interaction systems between diperoxovanadate complexes [OV(O2)2L]n- (n = 1 or 3, L = oxalate or H2O) and a series of histidine-like ligands in solution have been studied by using 1D multinuclear (1H, 13C, and 51V) NMR, 2D diffusion ordered spectroscopy, and variable-temperature NMR in 0.15 m...

The rapid increase in the prevalence of metabolic syndrome, which is associated with a state of elevated systemic oxidative stress and inflammation, is expected to cause future increases in the prevalence of diabetes and cardiovascular diseases. Oxidation of polyunsaturated fatty acids and sugars produces reactive carbonyl species, which, due to their electrophilic nature, react with the nucleo...

In the deoxy ferrous state of histidine-ligated heme proteins, the iron-histidine band (νFe-His) has been assigned as a stretching mode that involves a two-body motion involving the iron and histidine combined with a minor amount of heme doming. An analogous Raman band, νFe-L has been observed in the proximal cavity mutant of H93G myoglobin where the Raman band of a series of nonnative axial li...

The relationships of five feedback inhibitors for the Bacillus licheniformis glutamine synthetase were investigated. The inhibitors were distinguishable by differences in their competitive relationship for the substrates of the enzyme. Mixtures of l-glutamine and adenosine-5'-monophosphate (AMP) or histidine and AMP caused synergistic inhibition of glutamine synthesis. Histidine, alanine, and g...

The major pathway of histidine metabolism in animals and bacteria other than incorporation into protein involves first deamination to urocanic acid, then the anaerobic addition of water to form an intermediate generally considered to be 4(5)imidazolone-5(4)-propionic acid (1, 2). Finally, the ring is split enzymatically to ~formiminoglutamic acid (3) which is converted to free cglutamic acid by...