To examine the effect of pathogens on the diversity and structure of plant-associated bacterial communities, we carried out a molecular analysis using citrus and huanglongbing as a host-disease model. 16S rRNA gene clone library analysis of citrus roots revealed shifts in microbial diversity in response to pathogen infection. The clone library of the uninfected root samples has a majority of ph...

Diplogasteroides asiaticus n. sp. is described and illustrated, and its molecular profile and phylogenetic status within the family Diplogastridae are inferred. Morphologically, the new species is characterized by its stomatal structure, a tube-like stoma with three small, rod-like dorsal teeth and two subventral ridges; a spicule clearly ventrally bent at 1/3 from the anterior end; a gubernacu...

Citrus huanglongbing (HLB, ex greening) is one of the most serious diseases of citrus. Different forms of the disease are caused by different Candidatus Liberobacter species, Candidatus Liberibacter asiaticus (Las), Ca. L. africanus (Laf) and Ca. L. americanus (Lam). The pathogen is transmitted by psyllid insects and by budding with contaminated plant materials. The vector psyllid Diaphorina ci...

Hybridization in flowering plants depends, the first place, on delivery of pollen to a receptive stigma and subsequent growth tubes through style ovary, where sperm nucleus grain can ultimately fertilize egg cell. However, reproductive failure is often observed distant crosses caused by pre- and/or post-zygotic barriers. In this study, pre-fertilization barriers intertribal between Anemone coro...

This study reports the development of a loop-mediated isothermal amplification procedure (LAMP) for polymerase chain reaction (PCR)-based detection of 'Candidatus Liberibacter solanacearum', the bacterial causal agent of potato zebra chip (ZC) disease. The 16S rDNA gene of 'Ca. Liberibacter solanacearum' was used to design a set of six primers for LAMP PCR detection of the bacterial pathogen in...

Introduction Grass pea (Lathyrus sativus L.) is one of the most important species of the Lathyrus genus. It has two subspecies : spp. asiaticus Zalk., which has small, coloured seeds, occurring mainly in the continental part of Asia and Africa and spp. europaeus Zalk. with large seeds, occurring predominantly in Central Europe and European Russia. According to Vavilov (8) its genetic centre is ...