background: melanoma causes the greatest morbidity and mortality of all skin cancers. mucosal melanoma is a rare but highly aggressive neoplasm. according to previous studies the prevalence of kit mutations in acrallentiginous and mucosal melanomas is relatively low (less than 15–20%), but it can have profound therapeutic implications for localized high risk or metastatic diseases. our goal was...

introduction: the lymphatic system provides one of the main paths for the spread (metastasis) of cancer from one part of body to another. hodgkin’s diseases, lymphocytic leukemia, various metastatic disease and many lymph node disorders can be assessed by lymphoscintigraphy. radionuclide lymphoscintigraphy has been used for many years to define lymphatic drainage of melanoma. the most common ra...

Background and Aim: Implant site preparation with minimal trauma is an important factor in success of implant treatment and has a great effect on osseointegration. Piezoelectric bone surgery was introduced as a modern technique for micrometric osteotomy with precise, controllable action. Preservation of osteoblast cell viability is critical to achieve successful osseointegration. The present st...

The GeneChip 3’ IVT Express Kit is the newest option for generating target for Affymetrix 3’ expression arrays. The 3’ IVT Express Kit utilizes the same basic chemistry as GeneChip One-Cycle Target Labeling and Control Reagents, but employs a simplified workflow and optimized reagents that enable lower RNA input and increased ease of use. In this white paper, we describe the technical performan...

Hybridization-based target enrichment protocols require relatively large starting amounts of genomic DNA, which is not always available. Here, we tested three approaches to pre-capture library preparation starting from 10 ng of genomic DNA: (i and ii) whole-genome amplification of DNA samples with REPLI-g (Qiagen) and GenomePlex (Sigma) kits followed by standard library preparation, and (iii) l...

Following a nanopore sequencing run of PCR products of three amplicons less than 1kb, an abundance of reads failed quality control due to template/complement mismatch. A BLAST search demonstrated that some of the failed reads mapped to two different genes -an unexpected observation, given that PCR was carried out separately for each amplicon. A further investigation was carried out specifically...

Introduction: The lymphatic system provides one of the main paths for the spread (Metastasis) of cancer from one part of body to another. Hodgkin’s diseases, lymphocytic leukemia, various metastatic disease and many lymph node disorders can be assessed by lymphoscintigraphy. Radionuclide lymphoscintigraphy has been used for many years to define lymphatic drainage of melanoma. The most com...

This report describes the histological and immunohistochemical features of a small intestinal tumor that resembled a human gastrointestinal stromal tumor (GISTs) in a seven-year-old male cross-breed dog. This was unique because of the expression of desmin by the tumor. Grossly, the white-gray tumor measured 8.0 × 4.0 × 6.5 cm and was 760 gram in weight. It was cystic and enveloped the jejunum. ...