An Escherichia coli mutant, DF2000, accumulates up to 0.06 M glucose 6-phosphate when supplied glucose. This extremely large concentration of intracellular glucose 6-phosphate does not serve to induce the active transport of this ester. However, if the medium is then supplemented with only 5 X 1O-4 M glucose 6-phosphate the transport system is induced. Thus, it appears that exogenous glucose 6-...

The present experiments using primary cultures from renal proximal tubule cells examine two aspects of the regulation of sodium-dependent phosphate transport and membrane sodium-dependent phosphate transporter (Npt2a) expression by parathyroid hormone (PTH). Sodium-dependent phosphate transport in proximal tubule cells from wild-type mice grown in normal-phosphate media averaged 4.4 +/- 0.5 nmo...

The kinetics of transport and phosphorylation of the nucleoside analogue 5,6-dichloro-l-~-~-ribofuranosylbenzimidazole (DRB) and the nature of monophosphate metabolites obtained were studied in salivary gland cells of Chironomw tentans. DRB, labeled by exchange with tritiated water, is transported across the plasma membrane, but only monophosphate derivatives emerge in the ethanol-soluble cell...

Citation: Cosmidis J, Benzerara K, Guyot F, Skouri-Panet F, Duprat E, Férard C, Guigner J-M, Babonneau F and Coelho C (2015) Calcium-Phosphate Biomineralization Induced by Alkaline Phosphatase Activity in Escherichia coli: Localization, Kinetics, and Potential Signatures in the Fossil Record. Front. Earth Sci. 3:84. doi: 10.3389/feart.2015.00084 Calcium-Phosphate Biomineralization Induced by Al...

The urinary bladder of the fresh-water turtle acidifies its contents by actively transporting H(+) ions across the luminal membrane. It is known that the H(+) transport system is dependent upon oxidative metabolism and the substrate glucose; however, the specific biochemical events resulting in H(+) translocation have not been identified. This study examines the relationship between active H(+)...