p300/CBP-associated factor (PCAF) is a histone acetyltransferase that plays an important role in the remodeling of chromatin and the regulation of gene expression. It has been shown to catalyze preferentially acetylation of the epsilon-amino group of lysine 14 in histone H3. In this study, the kinetic mechanism of PCAF was evaluated with a 20-amino acid peptide substrate derived from the amino ...

A recombinant pig ribonuclease inhibitor (delta r-RI) lacking 90 or 93 N-terminal amino acid residues was isolated from a preparation of recombinant inhibitor. The kinetic parameters for the inhibition of ribonuclease A by delta r-RI were determined and found to be only slightly altered in comparison with the full-length inhibitor. The deletion did, however, affect the surface properties of RI....

Lysine-specific murine histone H3 methyltransferase, G9a, was expressed and purified in a baculovirus expression system. The primary structure of the recombinant enzyme is identical to the native enzyme. Enzymatic activity was favorable at alkaline conditions (>pH 8) and low salt concentration and virtually unchanged between 25 and 42 degrees C. Purified G9a was used for substrate specificity a...

Oral bacterial glucosyltransferases use sucrose as a substrate in synthesis of either alpha-1,3 water-insoluble glucans (GTF-I) or alpha-1,6 water-soluble glucans (GTF-S). The binding specificity of the glucosyl and fructosyl subsites of the sucrose-binding site was examined to identify ligands that bind exclusively to each subsite. Such compounds can be used as reporter ligands to localize the...

1. Human pancreatic juice contains amylase, lipase, ribonuclease, deoxyribonuclease, proelastase, procarboxypeptidase A, procarboxypeptidase B, chymotrypsinogen, and trypsinogen, as well as a tqqsin inhibitor. The level of the inhibitor is such that 1 ml of pancreatic juice containing 4 mg of protein will inhibit about 0.08 mg of bovine trypsin. The inhibitor is a small, basic protein, soluble ...

commercially available reduced NAP preparatioums for LDH assays. 1)ata on the influence of the inhibitor on otimer dehydrogenases are presented, as well as data concerning time inhibitor in coupled enzyme tests with dehydrogenases as indicator enzymes. Freshly prepareci solutions of various commercially available reduced NAT) preparations were foummd to l)e essentially free of inhibitors acting...

The proposed corrosion inhibition action of Acacia nilotica ethanol extract on aluminium in HCl and NaOH was investigated using weight loss method. found to be inactive at all the concentrations inhibitor but active with increasing efficiency as concentration decreases temperature increases highest 95.31%. kinetic thermodynamics data shows that adsorption process feasible endothermic from large...

The effect on enzyme kinetics of the presence of competitive inhibitors in constant molar ratio to t’he substrate has been described by Tubbs (I) and by Dalziel (2, 3). In this as yet unfamiliar situation, the inhibitor has the effect of increasing the apparent mutual affinity of enzyme and substrate. Thus, the slopes of reciprocal plots of the kinetic data are unchanged by the presence of the ...

The understanding of protein-ligand binding is of critical importance for biomedical research, yet the process itself has been very difficult to study because of its intrinsically dynamic character. Here, we have been able to quantitatively reconstruct the complete binding process of the enzyme-inhibitor complex trypsin-benzamidine by performing 495 molecular dynamics simulations of free ligand...