specificity is determined by the condensing enzyme, namely 8-ketoacyl-[acyl-carrier-proteinlsynthase 11. For the barley complex they are CoA derivatives which give rise to the C,,, C,, and C,, 3-oxoacyl key intermediates. One can speculate that the condensing enzyme activity specified by the cer-cqu-determined polypeptide is responsible for the noted substrate specificity. Therefore, should one...

Genotypic analysis of resistance to isoniazid (INH) in Mycobacterium tuberculosis is complex due to the various genes potentially involved. Mutations in ketoacyl acyl carrier protein synthase (encoded by kasA) were present in 16 of 160 (10%) INH-resistant isolates (R121K [n = 1], G269S [n = 3], G312S [n = 11], G387D [n = 1]). However, G312S was also present in 6 of 32 (19%) susceptible strains....

Ketoacyl reductases (KRs), hydroxyacyl dehydratases (HDs) and enoyl reductases (ERs) are part of the fatty acid and polyketide synthesis cycles. Their reverse reactions, catalyzed by acyl dehydrogenases (equivalent to ERs), enoyl hydratases (equivalent to HDs) and hydroxyacyl dehydrogenases (equivalent to KRs), are part of fatty acid degradation by β-oxidation. These enzymes have been classifie...

We have generated antisense plants of Brassica napus, targeting either the cytoplasmic Type I acetyl-CoA carboxylase or the beta-ketoacyl-acyl carrier protein reductase of the Type II dissociable fatty acid synthase, in order to investigate the importance of these components in regulating lipid metabolism in plants. Using a range of down-regulated plants, it has become clear that down-regulatio...

Cerulenin, [(2S,3R)-2,3-epoxy-4-oxo-7,10-dodecadienoylamide], a mycotoxin produced by Cephalosporium caerulens, irreversibly inactivated 6-methylsalicylic acid synthase from Penicillium patulum. A combination of radiolabelling studies with [3H]cerulenin, proteolytic and chemical digestion and N-terminal sequencing of labelled peptides indicated that the site of cerulenin modification is the hig...

Objective: The aim of the study is to perform a computational consisting molecular docking for polyphenols subjected in silico studies identify new lead antimicrobial activity which has been reported yet or not used yet. Methods: Schrödinger Maestro 11.3 performed enzyme FabH (β-ketoacyl-acyl carrier protein synthase III) (PDB ID: 5BNR) with polyphenol. targeted compounds were docked against an...