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Streptomyces strain K1-02, which was identified as a strain of Streptomyces albidoflavus, secreted at least six extracellular proteases when it was cultured on feather meal-based medium. The major keratinolytic serine proteinase was purified to homogeneity by a two-step procedure. This enzyme had a molecular weight of 18,000 and was optimally active at pH values ranging from 6 to 9.5 and at tem...

Microsporum canis is the main zoophylic dermatophyte in dogs and cats, and it is also an important zoonotic agent. The literature showed that cats are asymptomatic carriers of M. canis. This is apparently due to host resistance and/or the presence of strains with lower virulence. This study was aimed to evaluate the keratinolytic, elastinolytic and collagenolytic activities of M. canis strains ...

The root-galling index of tomatoes inoculated with Meloidogyne javanica was decreased 70% when collagen was used as a soil amendment (0.1% w/w) and 90% when the amendment was supplemented with the collagenolytic fungus Cunninghamella elegans. The root-galling index was reduced 80% when the fungus was homogenized in collagen culture medium and added to soil without collagen supplement. Culture f...

Dermatophytes are keratinolytic fungi responsible for a wide variety of diseases of the skin, nails and hair of mammals. Their identification is often complicated, labor-intensive and time consuming due to the high degree of intra-species morphological similarity, and also requires scientific knowledge and practice. The aim of this study was to demonstrate that MALDI-TOF MS technique may be a f...

Keratinases are proteolytic enzymes with a particular ability to cleave peptide bonds in keratin, and other proteins. Due their broad-spectrum of activity, keratinases considered viable substitutes for chemical thermal treatments protein-rich industrial by-products. Among these protein residues, special attention has been given keratinous materials (feathers, hair, horns, etc.), which disposal ...

The aim of this study was to evaluate peptidase production by Aspergillus terreus in solid-state bioprocess and evaluate its parameters. The best conditions were 5.0 g of wheat bran as substrate, incubation temperature 30°C, inoculum 2.0x10 spores/g and 75% saline volume, with production reaching 677 U/mL (5400 U/g culture medium) after 72 h of fermentation. Biochemical characterization of the ...

Keratinophilic fungi are natural colonizers of keratinic substrates. Some are keratinolytic and play an important ecological role in decomposing α-keratins, the insoluble fibrous proteins. Because of the tight packing of their polypeptide chains in α-helix structures and their linkage by disulphide bridges, they are poorly biodegradable. Two main forms of attack have been identified: surface er...