background and objectives: the aim of this study was to investigate the significance of multiple-mutations in the katg gene, predominant nucleotide changes and its correlation with high level of resistance to isoniazid in mycobacterium tuberculosis isolates that were randomly collected from sputa of 42 patients with primary and secondary active pulmonary tuberculosis from different geographic r...

In this study we aimed to learn about the nature and frequency of katG, inhA, and rpoB gene mutations underlying isoniazid (INH) and rifampin (RMP) resistance in clinical Mycobacterium tuberculosis complex isolates. The Silver Sequence DNA sequencing method was used to detect the resistance condition of 22 INH, 6 RMP, and 13 INH and RMP in previously determined drug-resistant clinical M. tuberc...

BACKGROUND Specific isoniazid (INH) resistance conferring mutations have been shown to impact the likelihood of tuberculosis (TB) transmission. However, their role in the clinical presentation and outcomes of TB has not been evaluated. METHODS We included all cases of culture-confirmed, INH monoresistant tuberculosis reported to the San Francisco Department of Public Health Tuberculosis Contr...

Studies carried out in recent years have established that growth under conditions of reduced gravity enhances Salmonella enterica serovar Typhimurium virulence. To analyze the possibility that this microgravity-induced increase in pathogenicity could involve alterations in the ability of Salmonella to withstand oxidative stress, we have compared the resistance to hydrogen peroxide of various Sa...

The oxidation of l-proline to glutamate in Gram-negative bacteria is catalyzed by the proline utilization A (PutA) flavoenzyme, which contains proline dehydrogenase (PRODH) and Δ(1)-pyrroline-5-carboxylate (P5C) dehydrogenase domains in a single polypeptide. Previous studies have suggested that aside from providing energy, proline metabolism influences oxidative stress resistance in different o...

The aim of this study was to investigate the significance of multiple-mutations in the katG gene, predominant nucleotide changes and its correlation with high level of resistance to isoniazid in Mycobacterium tuberculosis isolates that were randomly collected from sputa of 42 patients with primary and secondary active pulmonary tuberculosis from different geographic regions of Iran. Drug suscep...

Simultaneous expression of seven genes in Escherichia coli was measured by a reverse transcription-multiplex PCR fluorescence procedure. Genes studied were (i) oxyR (transcriptional regulator); (ii) katG, dps, gorA, and ahpCF (controlled by OxyR); (iii) sodA (controlled by SoxRS); and (iv) trxA (not related to OxyR or SoxRS). Except for trxA, transcription of all genes was activated during the ...

This study aims to evaluate the clinical value of PCR-fluorescent probes for detecting mutation gene associated with extensively drug-resistant tuberculosis (XDR-TB). The molecular species identification 900 sputum specimens was performed using polymerase chain reaction (PCR)–fluorescent probe. mutations drug resistance genes rpoB, katG, inhA, embB, rpsL, rrs, and gyrA were detected. convention...

BACKGROUND India has the highest estimated burden of tuberculosis in the world, accounting for 21% of all tuberculosis cases world-wide. However, due to lack of systematic analysis using multiple markers the available information on the genomic diversity of Mycobacterium tuberculosis in India is limited. METHODOLOGY/PRINCIPAL FINDINGS Thus, 65 M. tuberculosis isolates from New Delhi, India we...