The strain MA428-C1 was shaken-cultured (120 strokes/min., 8 cm amplitude) in. a medium (pH7.0) containing 2% glucose, 1.5% soybean meal, 0.3% NaCl, 0.1% K2HPO4, 0.1 % MgSO4-7H2O, 0.0007 % CuSO4-5H2O, 0.0001 % FeSO4-7H2O, 0.0008 % MnCl2-4H2O, 0.0002% ZnSO4-7H2O at 27°C for 5 days. The amount of gougeroxymycin was determined by a cylinder plate method described by one of the authors1}.. About 70...

Xylanases are hydrolytic enzymes produced by different microorganisms which convert xylooligomers to its constituent xylose units. The growing interests in xylanase production could be linked to its diverse industrial applications. The use of agricultural residues contributed in overcoming one of the major challenges of enzyme production, which is the production cost. This study involves the us...

Se realizó un estudio en el laboratorio de microbiología y biología la Universidad Nacional Huancavelica donde se aisló cultivó Nostoc sp. conocido como algas andinas (llullucha, yuyo, etc), teniendo objetivo conocer rendimiento biomasa tasa crecimiento in vitro esta especie. Las muestras fueron tomadas las lagunas Allccaccocha, Tutayacc Tansiriccocha (Distrito Nuevo Occoro); laguna Pachachaca ...

Cellulase finds use in saccharification of lignocellulosic agroresidues to fermentable sugars which can be used for production of commercially important metabolites. This study reports endoglucanase (CMCase) production by Enhydrobacter sp. ACCA2. The CMCase activity of the strain ACCA2 was successively improved by optimization of range of physical and nutritional parameter in a set of non-stati...

Papain was purified from spray-dried Carica papaya latex using aqueous two-phase system (ATPS). Then it was recovered from PEG phase by in situ immobilization or preparing cross-linked enzyme aggregates (CLEAs). The Plackett-Burman design and the central composite design (CCD) together with the response surface methodology (RSM) were used to optimize the APTS processes. The highly purified papa...

liquid chromatographic method was developed for the determination of pioglitazone in human plasma. After liquid–liquid extraction with diethylether, samples were quantitated on a NovaPak C8 column using a mixture of acetonitrile–140mM K2HPO4 (40:60, v/v, pH = 4.45) as mobile phase with UV detection at 269 nm. The flow rate was set at 1.4 mL/min. Ethylparaben was used as internal standard and th...

A Streptomyces strain MS-6-6 with promising anti-tuberculous activity was isolated from soil samples in Saudi Arabia. The nucleotide sequence of its 16S rRNA gene (1426 bp) evidenced a 100% similarity to Streptomyces mutabilis. Through an anti-tuberculous activity-guided approach, a polyketide macrolide was isolated and identified as treponemycin (TP). The structure of the isolated compound was...

Plackett-Burman design and Box-Behnken response surface methodology (RSM) was employed to optimize the medium components for the chitin deacetylase (CDA) activity from Bacillus amyloliquefaciens Z7. Plackett-Burman design was applied to determine the specific medium components affecting CDA activity and found that starch, chitin and MgSO4 were critical in augmenting CDA activity. These signific...