Lactate dehydrogenase isozyme 5 from rabbit skeletal muscle is activated by citrate, cis-aconitate, isocitrate, alpha-ketoglutarate, succinate, fumarate, malate, aspartate, and glutamate. In the presence of these activators the shape of the pyruvate saturation curve is changed from sigmoid to hyperbolic. Lactate dehydrogenase isozyme 1 from rabbit heart gives a hyperbolic pyruvate saturation cu...

Three cell lines (A.t. GRIP-1, 2, and 3) were established from Aedes triseriatus (Say) embryonated eggs or neonate larvae and their morphology, growth, karyotype, and isozyme pattern were studied. The isozyme alleles observed in the 3 cell lines also were found in adults of the original mosquito colony. Each cell line differed in enzymatic, morphological, and karyotypical patterns. La Crosse en...

Electrophoretic analysis of extracts of 12 bumblebee species revealed complex patterns of extramitochondrial diphosphopyridine nucleotide-dependent glycerol 3-phosphate dehydrogenase (EC 1.1.1.8) variants. Most Bombus species show one pattern, while Bombus nevadensis has a second and Bombus occidentalis a third pattern. All three Psithyrus (i.e. parasitic bumblebee) species tested have a fourth...

even purified enzyme preparations are often heterogeneous. for example, preparations of aspartate aminotransferase or cytochrome oxidase can consist of several different forms of the enzyme.  for this reason we consider how different the kinetics of the reactions catalysed by a mixture of forms of an enzyme must be to provide some indication of the characteristics of the species present.  based...

Glutamate dehydrogenase (GDH) has been purified from beef heart mitochondria and compared with crystalline beef liver GDH. The specific activity of heart GDH was 127 units and of liver GDH 80 units. Heart GDH subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis had a protein corresponding to liver GDH and a smaller molecular weight protein. On agarose gel electrophoresis heart...

Knüppel-Ruppert, Anja Sylvia, Gerolf Gros, Wolfgang Harringer, and Hans-Peter Kubis. Immunochemical evidence for a unique GPI-anchored carbonic anhydrase isozyme in human cardiomyocytes. Am J Physiol Heart Circ Physiol 278: H1335–H1344, 2000.—To clarify the controversial question of cell-specific distribution of carbonic anhydrase (CA) in the heart, endothelial cells and cardiomyocytes were iso...