objective: diabetes is an important risk factor for cardiovascular events. the great percent of morbidity in patients with diabetes is due to endothelial dysfunction. the present study investigated the effects of hydroalcholic extract of nigella sativa (n. sativa)  on contractile and dilatation response of isolated aorta in streptozotocin (stz)-induced diabetic rat. materials and methods: rats ...

The bulk volume (about 85%) of the mammalian liver parenchyma is contributed by the hepatocytes, whereas at least four other types of cells constitute the remainder (1). Procedures for the isolation of all five cell types have been described, although not from the same liver. However, the isolation of hepatocytes has clearly been the most widely used preparation and has proved extremely valuabl...

The objective of this study was to clarify the mechanism underlying hepatic uptake of dioscin (diosgenyl 2,4-di-O-a-L-rhamnopyranosyl-p-D-glucopyranoside), an herbal ingredient with antihepatitis activity, in rats and humans. The liver uptake index (LUI) in vivo, perfused rat liver in situ, rat liver slices, isolated rat hepatocytes, and human organic anion-transporting polypeptide (OATP)-trans...

The oxidation of ethanol by isolated liver cells from starved rats is limited by the rate of removal of reducing equivalents generated in the cytosol by alcohol dehydrogenase. Evidence is presented suggesting that, in these cells, transfer of reducing equivalents from the cytosol to the mitochondria is regulated by the intracellular concentrations of the intermediates of the malate-aspartate an...

The investigations described in this paper were undertaken as a preliminary to the study of the biochemical changes in the nucleus of the rat-liver cell during the induction of liver cancer. Allfrey (1954) and Stern & Mirsky (1953) described the isolation in aqueous media of nuclei from the thymus gland of the calf. Such preparations in vitro incorporated glycine and other amino acids into nucl...

The isolated perfused rat liver (IPRL) has proven to be a useful model for the study qf physiology and pathology of the liver, For research in nonparenchymal cell (NPC) function that includes measurement of cytokine production (eg, TNF), it is necessary to have a sterilized perfusion system, We have modified the IPRL apparatus so as to be able to perform sterile perfusions of two livers simulta...

Studies of isolated rat liver mitochondria were undertaken in order to evaluate the importance of glutamate transport, oxidation reduction state, and product inhibition on the rates of formation of ammonia from glutamate. Uptake and efflux of glutamate across the mitochondrial membrane were measured isotopically in the presence of rotenone. Efflux was stimulated by H+ in the mitochondrial matri...

Studies of isolated rat liver mitochondria were undertaken in order to evaluate the importance of glutama te transport, oxidation reduction state, and product inhibition on the rates of formation of ammonia from glutamate. Uptake and efflux of glutamate across the mitochondrial membrane were measured isotopically in the presence of rotenone. Efflux was stimulated by H+ in the mitochondrial matr...

Sublethal doses of E. coli endotoxin were shown to cause direct slowing of blood flow in the isolated perfused rat liver. Concentrations as low as SO ng per ml elicited this vasoconstrictive response. The decrease in flow after the initial lipopolysaccharide injection was immediate and lasted from. 5 to 15 minutes before recovering to baseline values. Succeeding injections of the same concentra...

1. The significance of the term nucleolus has been discussed. 2. A detailed method for the isolation of nucleoli from already isolated rat or cat liver nuclei has been presented. 3. The presence of DNA in isolated liver cell nucleoli has been indicated by histochemical methods. 4. The percentages of DNA and RNA in the isolated nucleoli have been determined by chemical analysis. 5. The specific ...