Shootin1 is a protein involved in neuronal polarization, and has been shown to be a key molecule for the positive/negative feedback loop for axon induction required during neuronal symmetry breaking. To better understand the molecular basis of shootin1 dynamics, we analysed the regulatory pathways and the expressional status of shootin1 gene during NGF-induced neuronal differentiation. We demon...

Mammalian AMP deaminase 3 (AMPD3) enzymes reportedly bind to intracellular membranes, plasma lipid vesicles, and artificial lipid bilayers with associated alterations in enzyme conformation and function. However, proteolytic sensitivity of AMPD polypeptides makes it likely that prior studies were performed with N-truncated enzymes. This study uses erythrocyte ghosts to characterize the reversib...

The mechanism of maternal mitochondrial DNA (mtDNA) inheritance in animals can be said to be the selective elimination of sperm mtDNA via the elimination factor of the egg and a sperm mitochondria-specific factor. In 2005, we clarified that t-tpis (Spag1 isoform 1) is a mitochondria-specific translocator and the sperm factor, and furthermore estimated that the elimination factors of the egg are...

Computational prediction of microRNA targets remains a challenging problem. The existing rule-based, data-driven and expression profiling approaches to target prediction are mostly approached from the gene-level. The increasing availability of RNA-seq data provides a new perspective for microRNA target prediction on the isoform-level. We hypothesize that the splicing isoform is the ultimate eff...

induced resistance to pathogen is one of the ways which plants use against biotic stresses. pathogenesis-related proteins(pr), including chitinase and β-1,3-glucanase synthesized by the plant in response to pathogenic infection. in this investigation, the expression of two isoforms: acidic and basic of chitinase and β-1,3-glucanasegenes were analyzed at 0, 6, 12, 24, 48 and 72 hours after funga...

purpose: neuromyelitis opitca (nmo) is an autoimmune disease that relates to deposition of anti-aquaporin-4 (aqp4) igg in the central nervous system (cns). however, called seronegative nmo when patients are negative for aqp4 antibody. nevertheless, nmo is most probably an antibody mediated disease. the aim of this study was to assess the association between seronegative nmo disease and variatio...

It is well-known that micromolar to millimolar concentrations of cardiac glycosides inhibit Na/K pump activity, however, some early reports suggested nanomolar concentrations of these glycosides stimulate activity. These early reports were based on indirect measurements in multicellular preparations, hence, there was some uncertainty whether ion accumulation/depletion rather than pump stimulati...

The canonical Wnt/β-catenin pathway is constitutively activated in more than 90% of colorectal cancer (CRC) cases in which β-catenin contributes to CRC cell growth and survival. In contrast to the Wnt/β-catenin pathway, the non-canonical Wnt pathway can antagonize functions of the canonical Wnt/β-catenin pathway. Wnt5a is a key factor in the non-canonical Wnt pathway, and it plays diverse roles...

PurposeAlternative ribonucleic acid (RNA) transcript splicing produces multiple RNA isoforms from the same gene which are associated with many heart diseases. However, little is known about alternative events in donor hearts during cold preservation.MethodsWe examined transcriptomic profile of left ventricle human comparing 0 and 8 hours preservation histidine-tryptophan-ketoglutarate solution....

Intracellular targeting may enable protein kinases with broad substrate-specificities, such as multifunctional Ca2+/calmodulin-dependent protein kinase (CaM kinase) to achieve a selectivity of action in vivo. We have examined the intracellular targeting of three delta-CaM kinase isoforms. The delta B-CaM kinase isoform is targeted to the nucleus in transfected cells while the delta A- and delta...