Bulk tank milk samples from 392 Northern Ireland dairy farms and individual animals (n = 293) on 4 of these were tested by a novel phagomagnetic separation (PhMS)-quantitative (q)PCR assay able to detect quantify viable Mycobacterium avium ssp. paratuberculosis (MAP), demonstrate its potential utility as surveillance tool. Viable MAP detected in 26.5% the bulk milks, with contamination levels r...

This study was conducted on dromedary camels suffering from disease suspected Johne’s disease. The clinical signs were chronic watery diarrhea, emaciation and decreased milk production. Inter-mandibular edema was noticed in two cases. Several investigations were performed, staining of intestinal smears with Ziehl–Neelsen stain, histopathology and PCR. Gross pathological examination revealed thi...

The aim of this study was to use PARACHEK Johne's Absorbed EIA-CSL, gamma interferon test IFN BOVIGAM-CSL , Mycobacterial Growth Indicator Tube MGIT, BACTEC 960 BECTON-DICKINSON, PCR with IS900 probe, to diagnose paratuberculosis in sheep from the Argentine North East (ANE) and compare the results with those of the comparative skin testing using bovine and avian PPD (B-PPD and A-PPD), agar gel ...

BACKGROUND The presence of Mycobacterium avium subspecies paratuberculosis (MAP) has previously been inferred in the genesis of Crohn's disease (CD), and a higher incidence of MAP PCR positivity has been demonstrated in the gut and peripheral blood of CD patients than in healthy individuals. The objective of this prospective study was to assess the potential etiological role of MAP in the patho...

INTRODUCTION Recent findings propose that Mycobacterium avium subsp. paratuberculosis (MAP) infection could act as risk factor in favoring multiple sclerosis (MS) progression. SLC11A1 is a gene associated with mycobacterial survival in the host and it may be involved in the induction and maintenance of autoimmune disease. METHODOLOGY In this preliminary study, 100 MS patients and 100 healthy ...

abstract        background and objective: paratuberculosis is a chronic granulomatous enteritis of ruminants caused by mycobacterium avium subspecies paratuberculosis (map). this study aimed to characterize the genome of the map 316f strain.       methods: the map 316f strain was subjected to the pcr-f57 and pcr-is900 experiments in order to ensure its identity as map. this was followed by appl...

Mycobacterium avium subspecies paratuberculosis (MAP) is a common pathogen in domestic ruminants that causes granulomatous inflammation of the small intestine leading to emaciation and wasting. Clinical disease (Johne's disease) is also reported for several wild ruminant species. Between 2007 and 2009 we collected 561 fecal samples from caribou (Rangifer tarandus ssp.) representing 10 herds of ...

Multitarget genotyping of the etiologic agent Mycobacterium avium subsp. paratuberculosis is necessary for epidemiological tracing of paratuberculosis (Johne's disease). The study was undertaken to assess the informative value of different typing techniques and individual genome markers by investigation of M. avium subsp. paratuberculosis transmission between wild-living red deer and farmed cat...

The role of mycobacteria, specifically Mycobacterium paratuberculosis, in Crohn's disease has aroused considerable controversy for many years. Using the ultra sensitive polymerase chain reaction some studies have reported detection of M paratuberculosis DNA in as many as 65% of Crohn's disease patients but also in patients without disease. Other studies have been negative for both groups. We th...

Reduced to near extinction in the late 1800s, a number of wood bison populations (Bison bison athabascae) have been re-established through reintroduction initiatives. Although an invaluable tool for conservation, translocation of animals can spread infectious agents to new areas or expose animals to pathogens in their new environment. Mycobacterium avium subsp. paratuberculosis, a bacterium tha...