Background: Parthenogenetic activation is a possible way to produce homogeneous embryos with the some ploidy. Probably such embryos could be used in other areas of biotechnology. Calcium signals are known as important regulators of oocyte activation. Extracellular calcium is required for initiation of meiotic resumption and development. Calcium ionophore A23187 is known to elevate intracellular...

Fragments of human lung parenchyma or bronchi were studied by high performance liquid chromatography, gas chromatography-mass spectrometry, and bioassay for the biosynthesis of 5-lipoxygenase metabolites of arachidonic acid, and by radioenzymatic assay for the release of histamine, upon immunologic and nonimmunologic stimulation. Human lung parenchyma were passively sensitized with serum from t...

The coagulant activity of tissue factor (TF) in the membranes of cultured cells is increased after physical disruption f the cells, and a similar increase can be elicited by treatment of cell cultures with calcium ionophore and calcium. We observed that the supernatants of cultures treated with calcium and ionophore CBI-A23187 contained more TF activity than those of control cultures. Phase con...

Intact bovine fibroblasts, pericytes, and kidney cells manifested significantly less tissue factor procoagulant activity than their disrupted counterparts. Addition of calcium ionophore A23187 rapidly and reversibly enhanced the cell-surface expression of tissue factor in intact cells up to the level achieved by disruption. Inhibitors of calmodulin blocked the ionophore-dependent enhancement of...

Photoresponsive ion extraction/release systems (PRIONERS) represent a highly interesting tool for the localized and time-controlled chemical perturbation of biological materials. We report here on our first results on phototriggered calcium and sodium exchanging materials. Such materials exist in two distinct states ("on" and "off"), depending on the wavelength of illumination. We used a combin...

The coagulant activity of tissue factor (TF) in the membranes of cultured cells is increased after physical disruption f the cells, and a similar increase can be elicited by treatment of cell cultures with calcium ionophore and calcium. We observed that the supernatants of cultures treated with calcium and ionophore CBI-A23187 contained more TF activity than those of control cultures. Phase con...