methods the current study generated two recombinant lentiviruses, plex-gcn and plex-gcp, bearing noxa and puma, respectively. transduction of both genes to hepatocarcinoma (hepg2) was verified using fluorescent microscopic analysis, western blotting, and quantitative real-time polymerase chain reaction (pcr). to evaluate the potential of noxa and puma to initiate apoptosis, a caspase-9 real-tim...

Imidazole-zinc reverse stain utilizing imidazole and zinc ions for protein visualization on electrophoresis gels was originally introduced in the 1990s (1–3). This method is based on the selective precipitation of imidazolate-zinc complex in the gel (3), except zones where proteins or other macromolecules, such as DNA (4–7) or lipopolysaccharide, are present (4,5,8,9). There are several advanta...

BACKGROUND ABCG2 is an ABC transporter. It has been demonstrated that endogenous ABCG2 expression in certain cancers is a possible reflection of the differentiated phenotype of the cell of origin and likely contributes to intrinsic drug resistance. But little is known about the contribution of ABCG2 to the drug resistance and the clinicopathological characteristics in breast cancer. In the pres...

Eleven children, eight females and three males, ranging in age from 17 months to seven years, eight months, with surgically corrected biliary atresia, were examined for oral problems. Four children had green staining of both p~’mary teeth and gingival tissues. Five children had abnormally large pulp chambers, and four had delayed primary tooth eruption. A significant relationship between primar...

background: subglottic stenosis is defined as inflammatory narrowing of the airway. it can be related to trauma, intubation, autoimmune disorders, vascular malformations, infection and congenital abnormalities. pot-wine stain (pws) is a small dermis venous malformation which is present at birth. pws is found on face and neck, but can occur in throat and can cause subglottic stenosis. case repor...

introduction: klippel–trenaunay syndrome (kts) is a rare congenital mesodermal abnormality characterized by varicose veins, cutaneous capillary malformation, as well as bone and soft tissue hypertrophy. case report: a 2-week-old female infant presented to our clinic because of vascular nevus and progressive enlargement of her right extremities and trunk since birth. the patient was treated with...

background:the present study aims to present a new method to trace narcotics in the solution extracted from dried urine stain left on cotton fabric. material and methods:narcotic substance was traced in the 50 samples through two methods. in the first method, samples were directly examined through thin layer chromatography (tlc). in the second method, a piece of cotton fabric was soaked in a be...

background: ct scan and nuclear medicine exams deliver a great part of medical exposures. this study examined professional radiation hazards in ct scan and nuclear medicine workers.   methods : in a cross sectional study 30 occupationally exposed workers and 7 controls (all from personnel of a laboratory) were selected. physical dosimetry was performed for exposed workers. blood samples were ob...

Salton (J. Gen. Microbiol. 30:223, 1963) summarized the evidence which demonstrates that the gram-positive cell is able to retain the crystal violet after iodine treatment because of the characteristic nature of its cell wall. This evidence is highly convincing, yet it does not explain the experiments of Henry and Stacy (Proc. Roy. Soc. London Ser. B 133:391, 1946) or of Bartholomew and Umbreit...