Although all species of the Brachypelma genus are protected under CITES, few studies have been performed on the genetic structure of the populations of these endangered tarantulas. Here we propose, for the first time in spiders, to use ISSR (Inter Simple Sequence Repeat) technique to study the genetic variability of Mexican populations of Brachypelma vagans (Ausserer 1875). We used a nonlethal ...

This study provides the first genetic characterization of the gypsy moth from China (Lymantriadispar), one of the most recognized pests of forests and ornamental trees in the world. We assessed genetic diversity and structure in eight geographic populations of gypsy moths from China using five polymorphic Inter simple sequence repeat markers, which produced reproducible banding patterns. We obs...

Hesperocyparis (Cupressus) goveniana and putative Cupressus goveniana subsp. gibsonensis plus H. pygmaea were analyzed by Inter-Simple Sequence Repeats (ISSRs). The ISSRs analyses support the continued recognition H. pygmaea and H. goveniana, but the recognition of Cupressus goveniana subsp. gibsonensis Silba was not supported. Phytologia 91(2):277-286 (August, 2009).

Genomic DNA extraction with a high quantity and quality is a basic requirement in molecular biology. The DNA obtained was free of any contamination proteins, polysaccharide, polyphenols and colored pigments. These compounds would interfere with the genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The isolated genomic DNA was fo...

Species identification is fundamentally important within the fields of conservation, biology, biogeography and ecology. Salvia species are herbaceous, biennial or annual, strongly aromatic. Inter-Simple sequence repeats (ISSR) molecular markers were used for evaluate genetic diversity relationship analysis 30 species. Ten selected ISSR primers amplified 116 loci, respectively, which all polymor...