The effect of pH, PO2, and inorganic phosphate on the uptake and metabolism of hypoxanthine by erythrocytes has been studied. Uptake of hypoxanthine and accumulation of inosine 5'-monophosphate (IMP) were markedly increased at acid pH, high external phosphate concentrations, and low PO2. Release of accumulated IMP as hypoxanthine occurred at alkaline pH values and low external phosphate concent...

Urinary inorganic phosphate excretion was studied before and during the administration of sodium bicarbonate and acetazolamide in dogs that were not given infusions of phosphate. The excretion fraction of filtered phosphate increased after sodium bicarbonate or acetazolamide was given. This phosphaturia was attributed to decreased tubular reabsorption of phosphate consequent to alkalinization o...

An isotopic shift of the (31)P nuclear magnetic resonance due to (18)O bonded to phosphorus of 0.0206 ppm has been observed in inorganic orthophosphate and adenine nucleotides. Thus, the separation between the resonances of (31)P(18)O(4) and (31)P(16)O(4) at 145.7 MHz is 12 Hz and, in a randomized sample containing approximately 50% (18)O, all five (16)O-(18)O species are resolved and separated...

The observed equilibrium constant for the hydrolysis of ATP to ADP and inorganic phosphate has been calculated as a function of pH and pMg ( -log (Mg2f)) at ZS”, along with the numbers of moles of H+ and Mg2+ produced per mole of ATP hydrolyzed. This equilibrium constant has also been calculated as a function of pMg and pCa (-log (Ca”+)) at a constant pH of 7.4, along with the numbers of moles ...

Experiments were conducted to determine whether en ergy charge, (ATP + 1/2 ADP)/(ATP + ADP + AMP) (in which AMP, ADP, and ATP are adenosine mono-, di-, and triphosphate, respectively) or intracellular inorganic phosphate was the primary regulator of the rate of aden osine 5 -monophosphate (AMP) catabolism in intact mouse ascites tumor cells. Hyperdiploid (ELD) and tetraploid (ELT/B1) cell suspe...

The synthesis of alkaline phosphatase by two strains of Vibrio cholerae belonging to the Inaba and Ogawa serotypes has been examined in relation to the phosphate concentration of the culture medium. The synthesis of the enzyme in both strains was repressed in cells grown in the presence of a high concentration of inorganic phosphate. Lowering the phosphate content of the growth medium led to a ...

Phosphate analyses are fundamental to a broad range of biochemical applications involving inorganic phosphate and organic phosphoesters such as phospholipids, phosphorylated proteins, and nucleic acids. A practical automated method utilizing robotics is described in this report. Five colorimetric methods of phosphate analyses based on formation of a phosphomolybdate complex and compatible with ...

Determination of inorganic phosphate is of very high importance in environmental and health care applications. Hence knowledge of suitable analytical techniques available for phosphate sensing for different applications becomes essential. Electrochemical methods for determining inorganic phosphate have several advantages over other common techniques, including detection selectivity, stability a...

Factors affecting candicidin synthesis and mycelial growth of Streptomyces griseus IMRU 3570 were studied. Inorganic phosphate was found to inhibit candicidin synthesis but to stimulate mycelial growth. Zinc, iron, and magnesium ions stimulated candicidin synthesis at relatively high concentrations in a complex medium but not in a synthetic medium. No other factors studied, such as temperature,...

BACKGROUND Recent studies demonstrate that interstitial inorganic phosphate is significantly elevated in the breast cancer microenvironment as compared to normal tissue. In addition it has been shown that breast cancer cells express high levels of the NaPi-IIb carrier (SLC34A2), suggesting that this carrier may play a role in breast cancer progression. However, the biochemical behavior of inorg...