Transcription associated with a terminal deoxynucleotide transferase gene initiator element is shown to respond to the transcription factor GAL4-VP16 both in vivo and in vitro. High-level transcription requires both an intact initiator element and bound activator. Transcription from this initiator-directed promoter is synergistic in vivo in that five GAL4 DNA binding sites yield 36 times the ex...

styrene polymerization through frp and atrp methods was carried out at 110oc, while in-depth studies were performed by monte carlo simulation.the changes in monomer conversion, initiator concentration, average molecular weight, and polydispersity index were computed over the course of the polymerization. as the results indicate, compared to atrp, the frp reaches higher conversion in a similar r...

Mixed-initiative in dialogue does not mean that either speaker is free to take the lead in the conversation at any time. Rather, who can show initiative, and when, is restricted. In this paper, we define initiative and control as two levels of dialogue phenomena, and give further evidence for our theory of restricted initiative that control belongs to the initiator of a discourse segment. We sh...

This article reviews various initiator used to synthesize high molecular weight (MW >10,000 g/mol) of polylactic acid (PLA) through ring-opening polymerisation (ROP) lactide. ROP has been chosen as the best method in producing PLA. On other hand, stannous octoate (Sn(Oct)2) reported catalyst for method. Many researchers have studied that rate with presence only Sn(Oct)2 is slow compared lact...

We have sequenced a methionine tRNA from mosquito mitochondria, and examined its structure using nucleases S1 and T1 under non-denaturing conditions. The sequence is highly homologous to a putative initiator methionine tRNA gene from Drosophila mitochondria. Its anticodon stem contains a run of three G-C base pairs that is characteristic of conventional initiator tRNAs; however, nuclease S1 ana...

A dencentralized consensus protocol refers to a process for all nodes in a distributed system to collect the information/status from every other node and reach a consensus among them. Two classes of decentralized consensus protocols have been studied before: the one without an initiator and the one with an initiator. While the one without an initiator has been well studied in the literature, it...

Baculovirus proteins P49 and P35 are potent suppressors of apoptosis in diverse organisms. Although related, P49 and P35 inhibit initiator and effector caspases, respectively, during infection of permissive insect cells. The molecular basis of this novel caspase specificity is unknown. To advance strategies for selective inhibition of the cell death caspases, we investigated biochemical differe...

Caspases execute cell death. The mechanism of effector caspase activation primarily involves reorganization of active site loops following the activation cleavage. The Induced Proximity hypothesis, originally proposed to explain the activation of initiator caspases, has recently been reinterpreted to be proximity-driven dimerization of initiator caspases, and consequently their activation. The ...