Recommended dietary reference intakes (DRI) for energy and nutrients for the Cuban population were first established by the Nutrition and Food Hygiene Institute (INHA, its Spanish acronym) in 1996.[1] International organizations and Cuban public health research subsequently generated a considerable volume of new information on food-based energy and nutrient requirements,[2-4] resulting in the n...

Tuberkulosis (TB) merupakan penyakit menular yang telah menginfeksi seperempat populasi dunia. Isoniazid adalah obat pilihan pertama untuk mengobati TB dengan mekanisme menghambat enoil ACP reductase (inhA) berperan penting dalam sintesis dinding sel M. tuberculosis. Akan tetapi, penggunaan isoniazid jangka relatif panjang dapat menghasilkan efek hepatotoksik sehingga diperlukan pengembangan an...

background: tuberculosis is one of the infectious diseases worldwide that has resurgence by the aids epidemic and led to the rise of drug-resistant tuberculosis patients. thus, it seems essential to monitor the drug susceptibility in tuberculosis patients. the new high resolution melting (hrm) method is simple, rapid and inexpensive for detection of the mutations responsible for drug resistance...

We reevaluated the BACTEC MGIT 960 antimicrobial susceptibility testing system (MGIT 960 AST) by using 1,112 isolates of Mycobacterium tuberculosis. When the results of MGIT 960 AST were compared with that of the proportion method using Ogawa medium (Ogawa PM), discrepant results were obtained for 30 strains with isoniazid, all resistant by MGIT 960 AST but susceptible by Ogawa PM. For 93% of t...

The enoyl-ACP reductase enzyme (InhA) from M. tuberculosis is recognized as the primary target of isoniazid (INH), a first-line antibiotic for tuberculosis treatment. To identify the specific interactions of INH-NAD adduct and its derivative adducts in InhA binding pocket, molecular docking calculations and quantum chemical calculations were performed on a set of INH derivative adducts. Reliabl...

The present study investigated the prevalence and diagnostic potential of the most commonly reported mutations associated with isoniazid resistance, katG 315Thr, katG 315Asn, inhA -15T, inhA -8A, and the oxyR-ahpC intergenic region, in a population sample of 202 isoniazid-resistant Mycobacterium tuberculosis isolates and 176 randomly selected fully sensitive isolates from England and Wales iden...