Multiple-target (multiplex) reverse transcription-PCR (RT-PCR) for detection, typing, and subtyping of the hemagglutinin gene of influenza type A (H3N2 and H1N1) and type B viruses was developed and applied prospectively to virological surveillance of influenza in England in the 1995-1996 winter season. During this season both influenza A H3N2 and H1N1 viruses were circulating, although at diff...

Two novel influenza A-like viral genome sequences have recently been identified in Central and South American fruit bats and provisionally designated "HL17NL10" and "HL18NL11." All efforts to isolate infectious virus from bats or to generate these viruses by reverse genetics have failed to date. Recombinant vesicular stomatitis virus (VSV) encoding the hemagglutinin-like envelope glycoproteins ...

BACKGROUND More and more nucleotide sequences of type A influenza virus are available in public databases. Although these sequences have been the focus of many molecular epidemiological and phylogenetic analyses, most studies only deal with a few representative sequences. In this paper, we present a complete analysis of all Haemagglutinin (HA) and Neuraminidase (NA) gene sequences available to ...

UNLABELLED H2 influenza viruses have not circulated in humans since 1968, and therefore a significant portion of the population would be susceptible to infection should H2 influenza viruses reemerge. H2 influenza viruses continue to circulate in avian reservoirs worldwide, and these reservoirs are a potential source from which these viruses could emerge. Three reassortant cold-adapted (ca) H2 p...

Influenza activity in the United States increased substantially during mid-December 1999 and appears to have peaked during the weeks ending December 25 (week 51) through January 15 (week 2). Predominant viruses isolated this season have been influenza type A(H3N2) viruses, antigenically similar to the viruses that have predominated since the 1997-98 influenza season and were well matched to thi...

Background and objective:Millions of people in developing countries lose their lives due to acute respiratory infections, such as Influenza A & B and Adeno viruses. Given the importance of rapid identification of the virus, in this study the researchers attempted to design a method that enables detection of influenza A, B, and adenoviruses, quickly and simultaneously. ...

BACKGROUND Type A influenza virus is one of important pathogens of various animals, including humans, pigs, horses, marine mammals and birds. Currently, the viral type has been classified into 16 hemagglutinin and 9 neuraminidase subtypes, but the phylogenetic diversity and distribution within the viral type largely remain unclear from the whole view. METHODOLOGY/PRINCIPAL FINDINGS The panora...

Background and Aims: Influenza A virus subtype H9N2 have continued to circulate in domestic poultry farms in Asia since 1998. The virus circulates in live bird markets, missing link in epidemiology of avian influenza. Regarding previous studies on H9N2 viruses of Iran and having no data on this subject in Iranian live bird markets this study was conducted to analyze genetically hemagglutinin pr...

one hundred sera from children 1 - 6 years of age, representative of a large serum collection, were tested for the prevalence of antibodies against different viruses. hemagglutination-inhibition (hi) antibodies were found in 68% for measles; 61 % for rubella; 75'% for influenza a2/hong kong/68, 16% for influenza b/md./59, 0% for group a arboviruses, 10% for group b arboviruses, 3% for phlebotom...