Aging cells not only cease growing, but also secrete various proteins such as inflammatory cytokines. This secretory phenomenon is known the senescence‑associated phenotype (SASP). The aim of present study was to elucidate effects senescence on differentiation osteoclast precursors (OCPs) and corresponding SASP. RAW264.7 were used OCPs cultured passage (P)5, P10 P20. Cell proliferation assays, ...

Murine macrophage death upon infection with Listeria monocytogenes was previously shown to be increased by beta interferon, produced by the infected cells. We saw that interferon-upregulated caspase activation or other interferon-inducible, death-associated proteins, including TRAIL, protein kinase R, and p53, were not necessary for cell death. Macrophage death was reduced when inducible nitric...

The expression of nitric oxide synthase (NOS) isoforms in the ovaries of pigs was examined to study the involvement of nitric oxide, a product of NOS activity, in the function of the ovary. Western blot analysis detected three types of NOS in the ovary, including constitutive neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS); eNOS immunoreactivity was more intense compared wi...

introduction: ischemia/reperfusion (ir) injury involves a complex interrelated sequence of events. high levels of nitric oxide (no) are generated with inducible form of nitric oxide synthase (inos) leading to the renal ir injury and glutathione (gsh) depletion. the present study was designed to investigate the effect of l-nil (n6- (1-iminoethyl)-l- lysine.hydrochloride), a selective inhibitor o...

The synthesis and biological evaluation of new types of N,N′-disubstituted thiourea and urea derivatives as inhibitors of both neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) are described. These compounds have been designed by reduction of the carbonyl group in the thiourea and urea kynurenamine derivatives 3 previously synthesized by our research group. The sy...

Lactic acid bacteria have become popular because of their γ-aminobutyric (GABA)-producing ability. In the present study, we selected four Levilactobacillus brevis strains (MG5552, MG5405, MG5261, and MG5522) with GABA-producing ability from 33 isolated various fermented foods in South Korea. We evaluated using thin-layer chromatography determined GABA levels produced by each strain an amino ana...

the genus artemisia is estimated to comprise over 800 species with anti-cancer, anti-fungal, anti-oxidant and anti-inflammatory properties. artemisia fragrans ( a. fragrans ), a species that belongs to genus artemisia , is rich in monoterpenes and sesquiterpenes derivatives. due to anti-inflammatory properties of monoterpenes and sesquiterpenes, we aimed to investigate the effect of a. fragrans...

Li, Jianrong, and Timothy R. Billiar. Nitric Oxide. IV. Determinants of nitric oxide protection and toxicity in liver. Am. J. Physiol. 276 (Gastrointest. Liver Physiol. 39): G1069– G1073, 1999.—Whereas nitric oxide (NO) produced by constitutive endothelial NO synthase is protective to the liver, NO produced by the inducible NO synthase (iNOS) can be either toxic or protective depending on the c...

Inducible nitric oxide synthase (iNOS) is responsible for nitric oxide (NO) synthesis from l-arginine in response to inflammatory mediators. It is reported that iNOS is degraded mainly by the ubiquitin-proteasome pathway in RAW264.7 cells and human embryonic kidney (HEK) 293 cells. In this study, we showed that iNOS was ubiquitinated and degraded dependent on CHIP (COOH terminus of heat shock p...

Exposure of mesangial cells to superoxide, generated by the hypoxanthine/xanthine oxidase system or by the redox cycler 2,3-dimethoxy-1,4-naphthoquinone caused a concentration-dependent amplification of interleukin (IL)-1beta-stimulated nitrite production. The effect of superoxide was accompanied by an increase in inducible nitric oxide synthase (iNOS) protein and iNOS mRNA levels. Incubation o...