An efficient plant regeneration method was established for Caesalpinia bonduc (L.) Roxb. by culturingimmature epicotyl explants. Morphogenic calli were initiated from 96% of epicotyl explants on MS mediumsupplemented with BA (6-benzylaminopurine) 4.0 mg/l and NAA (Naphthalene acetic acid) 1.0 mg/l. The calliformed were excised and subcultured on MS medium fortified with 1 mg/l...

In our studies on the development of new promoters for the root formation of tree cuttings, 4-trifluoromethylindole-3-acetic acid (4-CF(3)-IAA), a new fluorinated auxin, was synthesized via 4-trifluoromethylindole and 4-trifluoromethylindole-3-acetonitrile by using 2-methyl-3-nitrobenzotrifluoride as the starting material. As a control compound for comparing biological activities, 4-methylindol...

The first total synthesis of the mitotic spindle poison nostodione A is described. The inherent oxidative sensitivity of indoles is utilized for a late introduction of a second carbonyl to the cyclopent[b]indole-2-one system. The tricyclic system is prepared from indole-3-acetic acid and O-silylated 4-ethynylphenol, using a stereoselective intramolecular reductive Heck cyclization as the key tr...

An enzyme which catalyzes the conversion of L-tryptophan to indole-3-acetamide has been isolated from cells of Pseudomonas savastanoi and purified 89-fold. The enzyme catalyzes a reaction in which 1 mole of L-tryptophan and 1 mole of 02 are consumed and 1 mole of indole-3-acetamide and 1 mole of CO2 are produced. The enzyme has a pH optimum between 7.2 and 9.2. It is specific for the L isomer o...

Novel genes that function in the conversion of indole-3-acetamide (IAM) into indole-3-acetic acid (IAA), which were previously thought to exist only in the bacterial genome, have been isolated from plants. The finding of the AtAMI1 gene in Arabidopsis thaliana and the NtAMI1 gene in Nicotiana tabacum, which encode indole-3-acetamide hydrolase, indicates the existence of a new pathway for auxin ...

Bradyrhizobium japonicum E109 is a bacterium widely used for inoculants production in Argentina. It known its ability to produce several phytohormones and degrade indole-3-acetic acid (IAA). The genome sequence of B. was recently analyzed it showed the presence genes related synthesis IAA by indole-3-acetonitrile, indole-3-acetamide tryptamine pathways. Nevertheless, not able instead has this h...

Abbreviations: ACC: 1-Amino-cyclopropane-1-carboxylic acid ADC: arginine decarboxylase AgNO3: silver nitrate AVG: aminoethoxyvinylglycine BA: 6-benzylaminopurine DFMA: α-DL-difluromethyl arginine DFMO: α-DL-difluromethyl ornithine IAA: indole acetic acid NAA: α-naphthalene acetic acid ODC: ornithine decarboxylase PA: polyamine Put: putrescine SAM: S-adenosyl-Lmethionine STS: silver thiosulphate...

Recent experinm,ents with vertebrates (14, 17. 21. 36), insects (6, 10) and plants (2. 18. 19, 26, 28, 34) have shown that RNA and protein synthesis are involved in the action of a wide variety of hormones. In particular, the blockage of hormone action by low concentrations of the RNA polymerase inhibitor, actinomycin D, indicates a connection between hormone action and the de novo synthesis of...