The effect of cold storage of testes and epididymides at 4 °C for 12 h on the cryopreservation capacity of epididymal feline sperm was evaluated. Ten domestic cats were castrated, and testes and epididymides collected. Specimens were randomly assigned to two groups: in Group A, epididymal samples were immediately processed and frozen in 0.25-ml straws; in Group B, both testes and epididymides w...

BACKGROUND Near-infrared spectroscopy (NIRS) has the potential to continuously and noninvasively monitor intestinal function. This technology may be valuable because among neonates, intestinal maturity is highly variable and difficult to assess based solely on clinical signs. The aim of this study was to determine if there is an association between NIRS-based StO2 measurements and peristaltic a...

which could make parental consent growth enhancing. For example, since it favors old men with many adult daughters, it could raise longevity and population growth. Parental consent could also be important for understanding extended families. If extended families provide more household public goods, e.g. good health, this can counteract a less favorable allocation of private goods, e.g., human c...

OBJECTIVE Gallbladder stones are common in the Western world, and 70% to 80% of gallstones are cholesterol stones. This study investigates the effects of serum cholesterol, LDL, and HDL levels on gallstone cholesterol concentration. METHODOLOGY The gallstones of 75 patients with cholelithiasis were examined using spectrophotometry. RESULTS High serum cholesterol and LDL levels were associat...

OBJECTIVE To investigate, in men presenting with recurrent pregnancy loss (RPL), the prevalence of sperm autosome and sex chromosome aneuploidy. DESIGN Retrospective study. SETTING Male infertility clinic. PATIENT(S) A total of 140 men with RPL provided semen samples, and five normozoospermic controls provided 140 semen samples for comparison. Recurrent pregnancy loss, documented in the f...

This study determines the effect of different commercial diluents on quality frozen Simmental semen. The extraction fresh semen from 3 bulls using an artificial vaginal method was performed twice a week. Fresh with motility >70% further processed to one-step dilution method, and used were Andromed®, Optixcell®, Steridyl®. diluted packed into mini-straws, equilibrated, frozen, stored for test...

We report the development of a computerized, mathematical system for quantitating the various types of cell motility. This Fourier analysis method simultaneously quantifies for individual cells (i) temporal changes in cell shape represented by cell ruffling, undulation, and pseudopodal extension, (ii) cell translation, and (iii) average cell size and shape. This spatial-temporal Fourier analysi...

To evaluate and optimize the sperm separation efficiency of a novel silane-coated silica bead (Puresperm), serial studies were carried out to compare the various sperm parameters between: (i) three-layer (90%-70%-40%) Puresperm and three-layer (90%-70%-40%) conventional polyvinylpyrrolidone (PVP)-coated silica bead (Percoll) gradients; (ii) three-layer (90%-70%-40%) and two-layer (90%-45%) Pure...

background: it has been proposed that oxidative stress plays an important role in male infertility. the aims of this study were to compare seminal plasma levels of 15-f2t-isoprostane (8-iso-pgf2?), malondialdehyde (mda), and total (sum of free and bound) homocysteine (thcy) in normozoospermic vs. asthenozoospermic men, and to examine the relationships between thcy and lipid peroxidation product...

The motility of individual, aggregation-competent amebae of Dictyostelium has been analyzed at different concentrations of cAMP under both nongradient and gradient conditions. The following is demonstrated: (a) concentrations of cAMP greater than 10(-8) M inhibit motility in a concentration-dependent fashion, decrease the frequency but not the degree of turning, and cause rounding in cell shape...