A proficiency testing panel for detecting antibodies against Brucella abortus was developed and evaluated by both primary binding and conventional serological tests, using the guidelines of the World Organisation for Animal Health and the International Organization for Standardization Guide 43-1. All serological tests were judged satisfactory. Among the primary binding tests, the competitive en...

Amastigotes arc main pathogenic stage of I.eishmania parasite in mammalian host. During the course of leishmania infection the amastigotc surface would be covered by host proteins like immunoglobulins and complement components. These proteins produce false positive reaction and low signal/noise ratio in evaluation of antibody raising against lesion-dcrived amastigotc by indirect ELISA. 1'0 over...

Avian salmonellosis due to Salmonella B and D serogroups has a great importance because of the public health and the economical losses in poultry industry. For accurate diagnosis of avian salmonellosis, a total of 126 chicken sera evaluated with a commercial Salmonella Enteritidis (SE) ELISA kit have been used to develop two indirect ELISA models using lipopolysaccharide (LPS) and purified flag...

The apicomplexan parasite Theileria haneyi is one of two known causative agents equine theileriosis. It causes milder clinical disease than its more virulent counterpart, equi, in experimentally infected horses, and can superinfect T. equi-positive horses. current equi merozoite antigen 1 (EMA1)-based competitive enzyme-linked immunosorbent assay (ELISA)used the U.S. to detect theileriosis dete...

A new enzyme-linked immunosorbent assay (ELISA) respiratory syncytial virus antigen detection kit (Ortho Diagnostics, Inc., Raritan, N.J.) was compared with virus culture and with the indirect fluorescent antibody test (FAT) by using fresh nasal washings from children with suspected respiratory syncytial virus infection. Both uncentrifuged nasal washings and pellets from centrifuged split speci...