BACKGROUND Native immunoprecipitation followed by protein A-mediated recovery of the immuno-complex is a powerful tool to study protein-protein interactions. A limitation of this technique is the concomitant recovery of large amounts of immunoglobulin, which interferes with down-stream applications such as mass spectrometric analysis and Western blotting. Here we report a detergent-based "soft"...

Abstract Objective Retinoschisis and Norrie disease are X-linked recessive retinal disorders caused by mutations in RS1 NDP genes respectively. Both likely to be monogenic no locus heterogeneity has been reported. However, there reports showing overlapping features of retinoschisis a knock-out mouse model also the involvement both patients. Yet, exact molecular relationships between two have st...