We have measured thrombin-induced secretion of platelet factor 4 antigen (PF4) and simultaneously followed its intracellular translocation by immunofluorescence. In permeable resting platelets, speckled intracellular immunofluorescent staining for PF4 was observed. Addition of thrombin to washed platelets at 22 degrees C resulted in secretion of PF4 and formation of large (approximately 0.5 mic...

A novel method to monitor in situ hydrolyzable casein fragments during cheese ripening by using immunofluorescent labeling and confocal laser scanning microscopy (CLSM) was developed. Monoclonal single chain variable fragments of antibody (scFvs) were generated by antibody phage display toward three small synthetic peptides of the alpha(s1)-casein sequence. These peptides traverse enzymatic cle...

Rotavirus antisera with and without neutralizing activity to calf rotavirus were compared for their ability to agglutinate calf rotavirus particles with and without an outer capsid layer. Particles without the outer capsid layer were agglutinated by antisera with immunofluorescent antibody activity and by antisera with immunofluorescent antibody and neutralizing activity. Particles with the out...

L YMPHOID TISSUES of mice injected with Rauscher leukemia virus have been shown, by immunofluorescent technics, to contain antigen ( s ) reactive with antimurine leukemia antibody prepared against the Rauscher strain.’ Other immunofluorescent studies2’3 have demonstrated that blood and bone marrow cells from patients with leukemia, lymphoma, Hodgkin’s disease, polycythemia, teratoma, mycosis fu...

We report a case of classic Wegener's granulomatosis. Direct immunofluorescent study showed finely granular deposits of IgG and IgM in some of the alveolar walls, and of IgM in the maxillary sinus arteries. Electron-microscopic study of the lung and maxillary sinus showed intravascular fibrin, but failed to demonstrate electron-dense deposits in the blood vessel walls. Our patient also had circ...

Introduction: The implantation is a complex procedure that involves many molecules. One of these molecules is integrin specially &alphav&beta3 which serves as receptor for components of extra cellular matrix to act as bridging molecules between the blastocyst and the endometrial surface during the implantation process. By blocking &alphav&beta3 interactions, the implantation can be impaired. ...

L YMPHOID TISSUES of mice injected with Rauscher leukemia virus have been shown, by immunofluorescent technics, to contain antigen ( s ) reactive with antimurine leukemia antibody prepared against the Rauscher strain.’ Other immunofluorescent studies2’3 have demonstrated that blood and bone marrow cells from patients with leukemia, lymphoma, Hodgkin’s disease, polycythemia, teratoma, mycosis fu...

Indirect hemagglutinating and immunofluorescent antibody responses to Herpesvirus hominis types 1 and 2 were compared to neutralizing antibody responses in infected humans from whom H. hominis type 1 or 2 was isolated. The indirect immunofluorescent antibody test was shown to be the most sensitive and specific for primary human infections. The sensitivity and specificity of the indirect hemaggl...

INTRODUCTION The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells) must be competent to generate many thousands of differentiated neurons through asymmetric divisions during normal development. Studies in fly neuroblasts have been instrumental in identifying how the establishment and maintenance of cell p...

A new test for the detection of antibodies to gliadin in the sera of children who are gluten sensitive is described. This test is based on the observation that wheat protein binds selectively to connective tissue fibres in cryostat sections of mammalian tissues. Sera containing antibodies to gliadin give a reticulin pattern of staining on section pretreated with wheat gliadin if tested by indir...