نتایج جستجو برای: immobilized phanerochaete chrysosporium

تعداد نتایج: 18853  

Journal: :Applied and environmental microbiology 1994
C Mougin C Laugero M Asther J Dubroca P Frasse

Biotransformation of atrazine by the white rot fungus Phanerochaete chrysosporium was demonstrated by a 48% decrease of the initial herbicide concentration in the growth medium within the first 4 days of incubation, which corresponded to the mycelium-growing phase. Results clearly established the mineralization of the ethyl group of the herbicide. Analysis of the growth medium showed the format...

2015
Sean P. Gordon Elizabeth Tseng Asaf Salamov Jiwei Zhang Xiandong Meng Zhiying Zhao Dongwan Kang Jason Underwood Igor V. Grigoriev Melania Figueroa Jonathan S. Schilling Feng Chen Zhong Wang Deyou Zheng

Genes in prokaryotic genomes are often arranged into clusters and co-transcribed into polycistronic RNAs. Isolated examples of polycistronic RNAs were also reported in some higher eukaryotes but their presence was generally considered rare. Here we developed a long-read sequencing strategy to identify polycistronic transcripts in several mushroom forming fungal species including Plicaturopsis c...

2015
Aurélie Deroy Fanny Saiag Zineb Kebbi-Benkeder Nassim Touahri Arnaud Hecker Mélanie Morel-Rouhier Francis Colin Stephane Dumarcay Philippe Gérardin Eric Gelhaye Kristiina Hildén

White-rot fungi possess the unique ability to degrade and mineralize all the different components of wood. In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules. To cope with these molecules, wood decay fungi have developed a complex detoxification network including glutathione transferases (GST). The interactio...

Journal: :Applied and environmental microbiology 1998
B Li V Renganathan

Analysis of a 2.4-kb cDNA of the cellulose-binding extracellular beta-glucosidase (CBGL) from Phanerochaete chrysosporium suggested that CBGL is organized into two domains, an N-terminal cellulose-binding domain and a C-terminal catalytic domain. Genomic sequence analysis suggested that cbgl is encoded by 30 exons. Southern analysis of DNA from homokaryotic cultures indicated that CBGL is encod...

Journal: :Protein engineering, design & selection : PEDS 2013
Khajamohiddin Syed Aleksey Porollo David Miller Jagjit S Yadav

A promising polycyclic aromatic hydrocarbon-oxidizing P450 CYP5136A3 from Phanerochaete chrysosporium was rationally engineered to enhance its catalytic activity. The residues W129 and L324 found to be critical in substrate recognition were transformed by single (L324F) and double (W129L/L324G, W129L/L324F, W129A/L324G, W129F/L324G and W129F/L324F) mutations, and the engineered enzyme forms wer...

Journal: :The Journal of biological chemistry 2013
Miao Wu Gregg T Beckham Anna M Larsson Takuya Ishida Seonah Kim Christina M Payne Michael E Himmel Michael F Crowley Svein J Horn Bjørge Westereng Kiyohiko Igarashi Masahiro Samejima Jerry Ståhlberg Vincent G H Eijsink Mats Sandgren

Carbohydrate structures are modified and degraded in the biosphere by a myriad of mostly hydrolytic enzymes. Recently, lytic polysaccharide mono-oxygenases (LPMOs) were discovered as a new class of enzymes for cleavage of recalcitrant polysaccharides that instead employ an oxidative mechanism. LPMOs employ copper as the catalytic metal and are dependent on oxygen and reducing agents for activit...

1994
J. PIREZ

-Among 12 basidiomycetaceous fungal strains, Phanerochaete chrysosporium SC26 and K3 and Phanerochaete flavido-alba FPL 106507 were the most efficient in decolorizing paper mill wastes. Enzymatic activities attributable to both families of extracellular ligninases, lignin peroxidase (LIP) and manganese peroxidase (MnP), have been detected in P. flavido-alba FPL 106507. Both peroxidase activitie...

2012
Muhammad Asif Hanif Haq Nawaz Bhatti

Phanerochaete chyrsosporium is a white rot fungus with high heavy metal accumulating ability. In this regard, the present study was planned to assess the Ni (II) remediation potential of live immobilized Phanerochaete chyrsosporium from aqueous solutions as well as from real hazardous effluents. The effects of pH, dose, initial metal concentration, time, temperature etc. on bioremediation poten...

2001
Ming Tien

A cDNA clone encoding a manganese-dependent peroxidase from the filamentous fungus Phanerochaete chrysosporium was isolated and characterized. The clone, AMP-1, was isolated by screening a X g t l l expression library with polyclonal antibodies raised against a purified manganese-dependent peroxidase (isozyme H4, PI 4.5). The XMP-1 cDNA sequence predicts a mature protein containing 358 amino a...

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