As part of a research program aimed at producing biodiesel fuel from plant oils enzymatically cells of Rhizopus oryzae (R. oryzae) IFO4697 (with a 1,3-positional specificity lipase) immobilized within biomass support particles (BSPs) were investigated for the methanolysis of soybean oil. The R. oryzae cells easily became immobilized within the BSPs during batch operation. To enhance the methano...

Dextran a bacterial exopolysaccharide and a long chain polymer of glucose is produced by different microorganisms like Leuconostoc mesenteroides, Lactobacillus spp, Weissella spp and Streptococcus mutans. It has a wide range of application in the food, pharmaceutical and other industries and so is emerging as new and industrially significant product. In the present study the cells of Weissella ...

Pyocyanin is the blue phenazine pigment produced by Pseudomonas aeruginosa. Pyocyanin production using immobilized cells was investigated. The maximum production of pyocyanin was obtained using cells immobilized in kappa-carrageenan. Moreover, 0.01% PO4(3-), 0.2% Mg(2+), 0.001% Fe(2+), 1% glycerine, 0.8% leucine and 0.8% dl-alanine were also essential for pyocyanin production. Pyocyanin was pur...

New approaches for immunotherapy based on nanodiamond particles have been developed. The possible ways of conjugate synthesis comprising antibody as targeting vector, carrier, chlorine e6 photosensitizer, demonstrated. Spectral-luminescent properties the conjugates studied. It has shown that obtained effectively binds to cells in vitro. was observed immobilized ND reveals its photodynamic activ...

V. NAYDENOVA, V., S. VASSILEV, M. KANEVA and G. KOSTOV, 2013. Encapsulation of brewing yeast in alginate/ chitosan matrix: comparative study of beer fermentation with immobilized and free cells. Bulg. J. Agric. Sci., Supplement 2, 19: 123–127 In the present study, the possibilities for beer production with lager brewing yeasts, encapsulated in alginate/chitosan matrix with a liquid core, were i...

Cellular immunity was investigated in 43 patients with multiple myeloma (MM) by assessing ,HTdR uptake induced by monocyte-dependent [CD3 monoclonal antibodies (MoAbs), phytohemagglutinin (PHA)] and monocyte-independent (CD2 MoAbs, ionomycin + phorbolester) stimulations. The former were evaluated in peripheral blood mononuclear cells (PBMNC) and purified T cells; the latter were evaluated in pu...

The immobilization of cells can be defined as ‘‘the physical confinement or localization of cells to a certain defined region of space with preservation of some desired activity’’ (1). The region in which the cells are localized is called the immobilized cell system or immobilized cells aggregate, which can be divided into three components: the cells, the support (or carrier or matrix) material...

Cells o f Petroselinum crispum cv. “Paramount” and “Plain-leaved” immobilized in polyure­ thane foam retained the biotransformational characteristics o f their freely suspended cells for certain monoterpenic substrates. Immobilized cells o f both cultivars were more efficient than their free cells in isomerizing geraniol into nerol, but less efficient (<60% ) in reducing the alde­ hydes, citral...

Abstract Probiotic products require high number of viable and active microorganisms during storage. In this work, the survival human vaginal Lactobacillus gasseri CRL1320 rhamnosus CRL1332 after nanofiber-immobilization by electrospinning with polyvinyl-alcohol, storage was evaluated. The optimization bacterial immobilization conditions using bioprotectors (skim milk-lactose glycerol) oxygen-ex...