Some new 3-[(5-benzylidene-2-phenyl)-3, 5-dihydro-4-H-imidazol-4-one-3-(4-bezoylhydrazono)]-indole-2-ones (VIII) have been synthesized from different isatinhydrazones (II) by condensing with 2-phenyl-5-benzylidene- 3-N (4-acetyl phenyl)-1, 5-dihydro-imidazol-4-one (VII). Their chemical structures have been confirmed by IR, 1HNMR, MASS and by elemental analysis. Investigation of antimicrobial ac...

We have developed an algorithm for mapping proton wires in proteins and applied it to the X-ray structures of human carbonic anhydrase II (CA-II), the green fluorescent protein (GFP), and some of their mutants. For both proteins, we find more extensive proton wires than typically reported. In CA-II the active site wire exits to the protein surface, and leads to Glu69 and Asp72, located on an el...

This paper focuses on global gene regulation by FlhD/FlhC in enteric bacteria. Even though Yersinia enterocolitica FlhD/FlhC can complement an Escherichia coli flhDC mutant for motility, it is not known if the Y. enterocolitica FlhD/FlhC complex has an effect on metabolism similar to E. coli. To study metabolic gene regulation, a partial Yersinia enterocolitica 8081c microarray was constructed ...

P-glycoprotein (Pgp) is a membrane bound efflux pump spread in a variety of tumor cells and considered as a main component of multidrug resistance (MDR) to chemotherapies. In this work, three groups of compounds (imidazolone, oxazolone and vinyl dipeptide derivatives) were synthesized aiming to develop a molecular framework that effectively suppresses MDR. When tested for their influence on Pgp...

N-Formimino-n-glutamate is an intermediate in the degradation of n-histidine by such different types of cells as those of animal liver (1)) Pseudomonas jluorescens (2, 3), and Aerobacter aerogenes (3). The pathway leading from histidine to formiminoglutamate is the same in all cases and comprises three steps catalyzed by distinct enzymes: L-histidine is converted to urocanate and ammonia by his...

s: Five new mixed-ligand nickel (II) complexes; viz; [Ni (BHM)(PMDT)]1; [Ni(BHM)(dien)] 2; [Ni(BHM)(L 1 )]3; [Ni(BHM)(L 2 )] 4, [Ni(BHM)(L 3 )] 5; where H2BHM= N ’ -(1E)-(5-bromo-2-hydroxyphenyl) methylidene] benzoylhydrazide, PMDT= N,N,N’,N, ” N ” -Pentamethyldiethylenetriamine; dien= diethylenetriamine; L 1 = N,Ndimethyl-N’ (Pyrid-2-yl-methyl) ethylenediamine; L 2 = N-methyl-N’-(pyrid-2ylmeth...

BACKGROUND Abnormal hematocrit levels may interfere with glucose readings of patient self-assessment blood glucose (BG) meters. The aim of this laboratory investigation was to assess the potential influence of hematocrit variations on a variety of BG meters applying different measurement technologies. METHODS Venous heparinized blood was manipulated to contain three different BG concentration...

BACKGROUND There has been much recent interest in accumulation of advanced glycation end-products (AGE) in uraemic patients. Analysis of AGE has been difficult, because commonly used methodologies, i.e. immunodetection assays or fluorescence measurements, reflect group reactivity and are not specific for chemically defined substances. Some investigators measured individual AGE compounds, e.g. p...

The study is fo cused on the de ter mi na tion of the geo log i cal age of bi valve fauna re cov ered from four fossiliferous se quences in the Cacela For ma tion of Algarve, south ern Por tu gal, by means of the stron tium iso tope stra tig ra phy. Forty two anal y ses of bi valve sam ples from Barroquinha, Ca banas, the Cacela River and Lacem were per formed. The es ti mated age based on the ...