BACKGROUND Dastarcus helophoroides is known as the most valuable natural enemy insect against many large-body longhorned beetles. The molecular mechanism of its long lifespan and reproduction makes it a unique resource for genomic research. However, molecular biological studies on this parasitic beetle are scarce, and genomic information for D. helophoroides is not currently available. Thus, tr...

Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72). Its performance was tested by sequencing 2 exome captures, each consisting of 4 pre-capture pooled, barcoded Illumina libraries on an Illumina HiSeq 2500. At an average s...

BACKGROUND The emergence of next generation sequencing (NGS) has provided the means for rapid and high throughput sequencing and data generation at low cost, while concomitantly creating a new set of challenges. The number of available assembled microbial genomes continues to grow rapidly and their quality reflects the quality of the sequencing technology used, but also of the analysis software...

Multiplexing is a widely-used procedure that allows multiple DNA libraries to be pooled together for efficient sequencing. However, recent reports suggest that the DNA barcodes that label different libraries can “swap” on patterned flow-cell Illumina sequencing machines, including the HiSeq 4000, HiSeq X, and NovaSeq, thereby mislabelling molecules [1, 2]. This may compromise many types of -omi...

Colletotrichum musae is an important cosmopolitan pathogenic fungus that causes anthracnose in banana fruit. The entire genome of C. musae isolate GM20 (CMM 4420), originally isolated from infected banana fruit from Alagoas State, Brazil, was sequenced and annotated. The pathogen genomic DNA was sequenced on HiSeq Illumina platform. The C. musae GM20 genome has 50,635,197 bp with G + C content ...

Sugarcane (Saccharum spp. hybrid) is a noteworthy crop in the world for sugar and bioenergy production. In present study, transcriptomic analysis was steered high sucrose mutant Guixuan B9 (GXB9) low mother genotype of sugarcane during accumulating stage. A total 112,170 unigenes were obtained, 106,026 (94.52%) annotated by using available public databases nonredundant, Gene ontology, COG, Kyot...

The lignocellulosic enzymes of Trichoderma asperellum have been intensely investigated toward efficient conversion biomass into high-value chemicals/industrial products. However, lack genome data is a remarkable hurdle for hydrolase systems studies. secretory newly isolated T. ND-1 during lignocellulose degradation are currently poorly known. Herein, high-quality genomic sequence ND-1, obtained...

This report is the first to characterise the complete coding sequence of a dengue virus serotype 4 (DENV-4) genotype I that was isolated from field-caught mosquitoes from an endemic area in Thailand in June 2013. The sequence was assembled from high-throughput sequencing reads generated by Illumina HiSeq. Three out of four observed intra-sample variants caused an amino acid variation in C, NS2B...