An indirect enzyme-linked immunosorbent assay (I-ELISA) based on the recombinant VP3 protein of duck hepatitis A virus type 1 (DHAV-1) was developed and evaluated in this study. The optimal antigen, serum and enzyme-labeled antibody dilutions were 1:160 (0.94μg), 1:160 and 1:2000, respectively. The optimal blocking buffer was 1% gelatin. The cutoff value was determined to be 0.332, and the anal...

BACKGROUND Telomerase is a promising biomarker in cancer diagnosis and therapy. The elongation of telomeric repeats catalyzed by telomerase is accompanied by release of six PP(i) for each TTAGGG repeat (1 pmol PP(i)/310 pg telomeric repeats). We developed a novel method to measure telomerase activity by use of an enzymatic luminometric PP(i) assay (ELIPA). METHODS Extracts of cell lines and t...

BACKGROUND Control of brucellosis in livestock, wildlife and humans depends on the reliability of the methods used for detection and identification of bacteria. In the present study, we describe the evaluation of the recently established real-time PCR assay based on the Brucella-specific insertion sequence IS711 with blood samples from 199 wild boars (first group of animals) and tissue samples ...

Apoptosis of vascular smooth muscle cells (VSMCs) is of importance in the development of diabetic angiopathy. Our aim was to evaluate the effect of insulin and IGF-I on apoptosis in VSMCs. Rat aortic VSMCs were used and apoptosis was induced by serum starvation. As apoptotic markers we measured caspase-3 activity, histone-associated DNA fragments by ELISA and nuclear morphology by DAPI (4',6-di...

MJ Martin, Met Office, Exeter, UK. M Balmaseda, ECMWF, Reading, UK L Bertino, NERSC, Bergen, Norway P Brasseur, LEGI, Grenoble, France G Brassington, Bureau of Meteorology, Melbourne, Australia J Cummings, NRL, Monterey, USA Y Fujii, JMA/MRI, Tsukuba, Japan DJ Lea, Met Office, Exeter, UK J-M Lellouche, Mercator Ocean, Toulouse, France K Mogensen, ECMWF, Reading, UK P Oke, CSIRO, Hobart, Austral...

In this paper, we employ the view model given by Bertino to propose a new design approach for a secure multi-level object-oriented database system. The central idea is to provide the user with a multi-level view derived from a single-level secure object-oriented database. Hence the database operations performed on the multi-level views are decomposed into a set of operations on the single-level...

At ISPEC ’12, Samelin et al. show that the redactable signature scheme introduced at VLDB ’08 by Kundu and Bertino does not always preserve the structural integrity of the tree signed. In particular, they show how redaction of non-leaves promotes descendants and allows a third party to add new edges to the signed tree. This alters the semantic meaning of the tree and is not acceptable in certai...