The effects of osmotic stress on chloride (CI-) currents in the human neuroblastoma cell line CHP-100 were evaluated. Following exposure to hypoosmotic solution, an increase in whole-cell CI- current was observed. This current was blocked by the CI- channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB). In cells loaded with the CI- permeability marker 125I, exposure to hypoosmotic...

Fresh semen obtained from infertile men occasionally exhibits the total absence of motile sperm (100%asthenozoospermia). Vitality tests may reveal that a proportion of the immotile sperm has functional membranes, which are considered viable. An easy test for the prior determination of sperm viability may assist in sperm selection for the microinjection of oocytes. The main objective of this stu...

Articular chondrocytes in osteoarthritis (OA) patients are exposed to hypoosmotic stress because the osmolality of this synovial fluid is significantly decreased. Hypoosmotic stress can cause an efflux of Cl(-) and an associated decrease of cell volume. We have previously reported that a Cl(-) conductance contributes to the regulation of resting membrane potential and thus can alter intracellul...

The inositol phospholipids phosphatidylinositol, phosphatidylinositol 4-phosphate (PIP), and phosphatidylinositol 4,5-bisphosphate (PIP2) comprise 14.8, 1.2, and 0.3 mol %, respectively, of Dunaliella salina phospholipids. In isolated plasma membrane fractions, PIP and PIP2 are highly concentrated, together comprising 9.5 mol % of plasmalemma phospholipids. The metabolism of these inositol phos...

this study aimed at determining the physico-morphological attributes, in vitrofertilityand their interrelationships in semen of sahiwal bulls. semen samples werecollected weekly for 6 weeks from ten mature sahiwal bulls divided randomly into 5 groups. the mean (±sem) of ejaculate volume (ml), mass activity (score 0-5), progressive sperm motility (%), sperm concentration (106/ml), live sperm (%)...

We used fura-2 video imaging to characterize two Ca2+ influx pathways in mouse thymocytes. Most thymocytes (77%) superfused with hypoosmotic media (60% of isoosmotic) exhibited a sharp, transient rise in the concentration of intracellular free Ca2+ ([Ca2+]i). After a delay of approximately 70 s, these swelling-activated [Ca2+]i (SWAC) transients reached approximately 650 nM from resting levels ...

soil is known as the base material for many structure, on the other hand, some soils cause problems for some structures such as roads. some types of soils absorb moisture and swell then they may shrink when losing water. the changes of moisture in these soil samples cause changes their volume and pressure to layers of the pavement and eventually leads to destruction of roads. therefore, it is n...