The influence of electric field treatment on dedifferentiation and calli formation on rape hypocotyls was investigated. Segments, 10 mm long, of the upper part of rape (Brassica napus L., cv. Góczański) hypocotyls were stimulated by different combinations of voltage/time (1.5 V/120 h, 3 V/3 h, 10 V/15 min and 30 V/30 s) under in vitro conditions. With all electric field treatments, segments ori...

Hazelnuts are usually propagated by suckers or layering. However, other methods of propagation have been tested with variable results. Grafting is a method that has a few advantages such as fast multiplication rate, earlier fruiting and reducing sucker removal cost on specific rootstocks. Unfortunately, grafting is difficult due to slow formation of callus in hazelnuts. Here, we report the hypo...

hazelnuts are usually propagated by suckers or layering. however, other methods of propagation have been tested with variable results. grafting is a method that has a few advantages such as fast multiplication rate, earlier fruiting and reducing sucker removal cost on specific rootstocks. unfortunately, grafting is difficult due to slow formation of callus in hazelnuts. here, we report the hypo...

Phenylalanine ammonia-lyase activity increased rapidly beginning 2 h after inoculation with Phytophthora megasperma (Drechs.) f.sp. glycinea (Hildeb.) Kuan & Erwin race 1 in unwounded hypocotyls of soybean cv. Harosoy 63 (resistant) but did not change significantly in cv. Harosoy (susceptible). Small increases in phenylalanine ammonia-lyase activity also were caused by wounding. Activity increa...

Phytochrome belongs to red/far-red light family of photoreceptors. It exists in two spectral forms named red absorbing form (Pr), said be the inactive and far-red (Pfr) which is active form. This photoreceptor structurally divided into modules- amino acid (N-) terminal photosensory module caboxylic (C-) His kinase-like catalytic output module. Five different types exist Arabidopsis (PhyA-E). Ro...

Intestinal explants are a useful model for toxicological and pathological studies, but the maintenance of tissue viability for longer periods of incubation remains challenging. The aim of this study was to evaluate the effect of age on the viability of intestinal explants. Jejunal explants were obtained from 24and 42-day-old piglets. The explants were incubated for two or four hours and were th...

An efficient protocol for callus induction and somatic embryogenesis of Cyclamen premium Mill. Was developed. In this study we investigate difference between response of two explants type leaf and petiole and two medium salt concentrations MS and 1⁄2 MS on callus percentage and somatic embryo number. Media was non-significant on callus production but leaf explants was better than petiole and in...