1. A tissue culture method was devised in which suspensions of osteoblasts, obtained directly from frontal bones of fowl embryos, were grown in a fluid, fibrin-free medium. 2. Maximum growth of the tissue, as measured by dry weight, with the formation of collagen protein, based on the estimation of hydroxyproline, was obtained in periods of up to 6 days. 3. Appreciable amounts of protein-bound ...

To study qualitative and quantitative relations between proliferation of bile ductules and hepatic fibrogenesis, experimental hepatic fibrosis was produced in rats by subacute ethionine intoxication, bile duct ligation, administration of a-naphthylisothiocyanate, and by a high fat-low protein diet. Regression of fibrosis was produced in ethionine-intoxicated animals by treatment with methionine...

HYPOTHESIS Anti-inflammatory corticosteroids significantly impair wound healing. Retinoids partially, but significantly, reverse this effect. Little is known about the mechanism of steroid retardation or retinoid reversal. We hypothesized that corticosteroids lower transforming growth factor-beta (TGF-beta) and insulin-like growth factor-I (IGF-I) levels and tissue deposition in wounds and that...

The ethanolic extract of S. robusta resin (10 and 30 % w/w applied locally in excised and incised wounds) produced a dose-dependent acceleration in wound contraction and increased hydroxyproline content and tensile strength of wounds in rats. The results demonstrate wound healing activity of ethanolic extract of S. robusta resin.

1. Male rats of approximately 120 g body-weight were maintained on a commercial stock diet containing 204 g crude protein (nitrogen x 6.25)/kg, a hydroxyproline-free high-protein (HP) diet containing 200 g casein/kg as the only protein source, or a low-protein (LP) diet containing 40 g casein/kg. After 6 weeks on these diets half of each group was transferred to a non-protein (NP) diet and the ...

Early reperfusion after a coronary occlusion may reduce myocardial infarct size, but late reperfusion into necrotic myocardium may alter post-infarction healing. In rabbits, we compared 1- or 3-week-old scars resulting from permanent coronary occlusion to those resulting from a 1- or 3-hour occlusion followed by reperfusion. Reperfusion at 1 hour post-occlusion did not affect scar mechanical pr...