Abstract Polylactic acid (PLA) and poly(3-hydroxybutyrate- co -4-hydroxybutyrate) (P(3HB- -4HB)) nanofibers were prepared by melt extrusion of immiscible blends PLA/polyvinyl alcohol (PVA) P(3HB- -4HB)/PVA via in situ formation microfibrils during the process. The morphology after removal PVA with water was studied using scanning electron microscopy. intermolecular interactions Fourier-transfor...

ABSTRACT: Patients with acute renal failure often have a negative energy balance, which promotes metabolic changes predisposing to complications. The objective of this study was evaluate laboratory parameters 30 dogs severe failure, assess their relationship the possibility developing pancreatitis due and correlate these findings degree failure. Serum concentrations urea, creatinine, beta-hydro...

Concentrations of acetoacetate and P-hydroxybutyrate and their specific radioactivities were shown to be assayable directly in plasma of the rat without the need for prior deproteinization. By utilizing this technique ketone body turnover was estimated in the isotopic steady state and after the single injection of radioactive acetoacetate or P-hydroxybutyrate. While difEculties exist in both ty...

Genes encoding 3-hydroxybutyrate oligomer hydrolase (PhaZc) and 3-hydroxybutyrate dehydrogenase (Hbd) were isolated from Paracoccus denitrificans. PhaZc and Hbd were overproduced as His-tagged proteins in Escherichia coli and purified by affinity and gel filtration chromatography. Purified His-tagged proteins had molecular masses of 31 kDa and 120 kDa (a tetramer of 29-kDa subunits). The His-ta...

PURPOSE Retinal adenosine triphosphate is mainly produced via glycolysis, so inhibition of glycolysis may promote the onset and progression of age-related macular degeneration (AMD). When glycolysis is inhibited, pyruvate is metabolized by lactic acid fermentation instead of entering the mitochondrial tricarboxylic acid (TCA) cycle. We measured urinary pyruvate and lactate levels in patients wi...

Glucagon has been shown previously to increase further the enhanced tolerance for hypoxia observed in mice with elevated blood ketones. Glucagon is also known to increase blood glucose and to alter directly the metabolism of some (liver) cells. Both the increase in blood glucose and altered cellular metabolism could contribute to the increase in tolerance for hypoxia observed in mice given gluc...

beta-hydroxybutyrate and acetoacetate as well as lactate and pyruvate are intermediary metabolites normally present in blood. The beta-hydroxybutyrate/acetoacetate ratio is an expression of the mitochondrial oxido-reduction state. This ketone body ratio can provide a clue to diagnosis and metabolic status in congenital errors of the electron transport chain and pyruvate metabolism. The standard...

Alicycliphilus denitrificans strain BC grows anaerobically on acetone with nitrate as electron acceptor. Comparative proteomics of cultures of A. denitrificans strain BC grown on either acetone or acetate with nitrate was performed to study the enzymes involved in the acetone degradation pathway. In the proposed acetone degradation pathway, an acetone carboxylase converts acetone to acetoacetat...

The design of new synthetic grafted poly(3-hydroxybutyrate) as composite 3D-scaffolds is a convenient alternative for tissue engineering applications. The chemically modified poly(3-hydroxybutyrate) is receiving increasing attention for use as biomimetic copolymers for cell growth. As of yet, these copolymers cannot be used efficiently because of the lack of good mechanical properties. Here, we...