Enzymes can catalyze various reactions with high selectivity and are involved in many important biological processes. However, the general instability of enzymes against high temperature often limits their application. To address this, we synthesized a trehalose-based hydrogel in two steps from commercial starting materials with minimal purification procedures. Mono- and multi-functional trehal...

A novel cross-linked honey hydrogel dressing was developed by incorporating Malaysian honey into hydrogel dressing formulation, cross-linked and sterilized using electron beam irradiation (25 kGy). In this study, the physical properties of the prepared honey hydrogel and its wound healing efficacy on deep partial thickness burn wounds in rats were assessed. Skin samples were taken at 7, 14, 21,...

Tissue adhesion is a common complication after surgery. In this work, a dexamethasone loaded polymeric micelles in thermosensitive hydrogel composite (Dex hydrogel) was prepared, which combined the anti-adhesion barrier with controlled release of anti-adhesion drug. Dexamethasone (Dex) was encapsulated in polymeric micelles (Dex micelles), and then the Dex micelles were loaded into biodegradabl...

Cell-based biosensors have become a research hotspot in biosensors and bioelectronics fields. The main feature of cell-based biosensors is the immobilization of living cell on the surface of transducers. Different types of polymers which are used as scaffolds for cell growth should be biocompatible and have reactive functional groups for further attachment of biomolecules. In this work, the cel...

Responsive hydrogels functionalized with molecular recognition agents can undergo large volume changes upon interactions with specific chemical species. These responsive hydrogels can function as chemical sensing materials if the hydrogel volumes are monitored by using devices such as photonic crystals (PhC). An important criterion of merit is the responsiveness of these sensing hydrogels. Gene...

Development of a biomimetic 3D culture system for drug screening is necessary to fully understand the in vivo environment. Previously, a self-assembling peptide hydrogel has been reported; the hydrogel exhibited physiological properties superior to a 3D cell culture matrix. In this work, further research using H9e hydrogel with HeLa cells was carried out considering H9e hydrogel's interaction w...

This paper reports a new approach for constructing blood vessel-like tissues using microfluidic devices made of calcium-containing agarose hydrogel. Sodium alginate (NaA) solutions with different cell types at high densities were introduced stepwise into the hydrogel microchannel, to deposit cell-containing multiple Ca-alginate hydrogel layers on the channel surface with the help of Ca 2+ ions ...

In this study, several examples of hydrogels and nanocomposite hydrogels based on PVP with different content of montmorillonite nanoclay were prepared. Then, the swelling of hydrogels and kinetics of drug delivery of hydrogel in an environment similar to the body (pH 7.4) were examined. The effect of nanoparticle different percentages on the hydrogel was clearly observed. Then kinetics of drug ...

Bottom-up approach is a potentially useful tool for hydrogel assembly of cell-laden individual building blocks. In this article, we assembled individual building blocks of photocrosslinkable microgels in a rapid and controlled manner. Individual building blocks of poly(ethylene glycol) (PEG) microgels with square and hexagonal shapes were fabricated by using a photolithography technique. Indivi...