The complex of hyaluronic acid (HA)-binding region, link protein, and hyaluronic acid was isolated from trypsin digests of bovine nasal cartilage proteoglycan aggregates and the HA-binding region was then purified from the complex. After dansylation of accessible amino groups, less than 15% of the HA-binding region molecules interacted with hyaluronic acid. Conversely, after acetylation with 2....

Objective(s):Non-alcoholic steatohepatitis (NASH), is an important component of Non-alcoholic fatty liver disease (NAFLD) spectrum, which progresses to the end stage liver disease, if not diagnosed and treated properly. The disproportionate production of pro- and anti-inflammatory adipokines secreted from fat contributes to the pathogenesis of NASH. In this study, the comparative effect of piog...

Poly-L-lactic acid (PLLA) electrospun nanofiber scaffold is one of the most commonly used synthetic polymer scaffolds for bone tissue engineering application. However, PLLA is hydrophobic in nature, hence does not maintain proper cell adhesion and tissue formation, moreover, it cannot provide the osteo-inductive environment due to inappropriate surface characteristic and the lack of surface mot...

A complex consisting of the hyaluronic acid-binding region of proteoglycan molecules, the link protein, and hyaluronic acid was purified from trypsin digests of aggregates isolated from the Swarm rat chondrosarcoma. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis showed that the molecular weights for the hyaluronic acid-binding region and the link protein were about 67,000 and 4...

The protein-keratan sulfate core of bovine nasal cartilage proteoglycan was purified by affinity chromatography on a column of immobilized hyaluronic acid. The hyaluronic acid was immobilized by reaction with a hydrazido-alkyl derivative of Sepharose in the presence of borohydride. Proteoglycan was digested with chondroitinase ABC and the entire mixture was passed over a column of the Sepharose...

objective(s): protein kinase c (pkcα) is involved in modulating articular chondrocytes apoptosis induced by nitric oxide (no). hyaluronic acid (ha) inhibits nitric oxide-induced apoptosis of articular chondrocytes by protecting pkcα, but the mechanism remains unclear. the present study was performed to investigate the effects and mechanisms of pkcα regulate protective effect of hyaluronic acid....

One of key factors affecting the effective application of tissue engineering is the development of scaffolds, which provide the physical support for adhesion and expansion of cells that can regenerate the lost of diseased tissue. Recently, several scaffolds consisting of synthetic materials or substance derived from natural tissue, such as poly(lactic acid) (PLA) (1), poly(glycolic acid) (PGA) ...

BACKGROUND A high concentration of hyaluronic acid in pleural fluid is suggestive of malignant mesothelioma. However, a relatively high concentration of hyaluronic acid was also seen in the pleural fluid of patients with benign inflammatory diseases. To show the utility of measuring hyaluronic acid levels in pleural fluid to diagnose tuberculous pleurisy, we compared the clinical features and l...

It has previously been shown that in most joint diseases changes occur in the hyaluronic acid in the joint fluid. In diseased fluids the hyaluronic acid has a considerably lower degree of polymerization than in normal fluids or in uncomplicated traumatic effusions (Sundblad, 1953). The hyaluronic acid changes are especially marked in rheumatoid arthritis. We recently demonstrated (Sundblad, Ege...