Interleukin-1beta (IL-1beta) stimulates expression of the highly inducible enzyme cyclooxygenase-2 (COX-2) via activation of nuclear factor kappaB (NFkappaB), and consequently provokes prostaglandin E(2) (PGE(2)) synthesis, which induces inflammatory responses. In this study, the contribution of protein kinase C (PKC) to IL-1beta-induced PGE(2) synthesis in human gingival fibroblasts was invest...

Cystein proteinases (gingipains) from Porphyromonas gingivalis cleave a broad range of in-host proteins and are considered to be key virulence factors in the onset and development of adult periodontitis and host defense evasion. In periodontitis, an inflammatory disease triggered by bacterial infection, the production of hepatocyte growth factor (HGF) is induced not only by various factors deri...

Neovascularization is one of the most important processes during tissue repair and regeneration. Current healing approaches based on the use of biomaterials combined with stem cells in critical-size bone defects fail due to the insufficient implant vascularization and integration into the host tissues. Therefore, here we studied the attraction, ingrowth, and distribution of blood vessels from t...

background and aims. the aim of this study was to evaluate the effect of dilution and curing methods of an etch-and-rinse adhesive and a self-etching primer from the same manufacturer at early exposure time on cytotoxicity of primary hu-man gingival fibroblasts. materials and methods. primary human gingival fibroblasts were exposed to different dilutions of adper single bond (asb) and adper pro...

BACKGROUND AND AIM: An important goal of periodontal plastic surgery is the creation of attached gingiva around the teeth. In this study, the aims were to culture gingival fibroblasts in a biodegradable scaffold and measure the width of attached gingiva after the clinical procedure.METHODS: This study was carried out on 4 patients (8 sites), with inadequate attached gingiva next to at least two...

In a previous study, we demonstrated that the amount of interleukin (IL)-8 mRNA expressed by human gingival fibroblasts stimulated with lipopolysaccharide (LPS) from Prevotella intermedia ATCC 25611 is increased by pre-treatment with beta or gamma interferon (IFN-P or -y). In the present study, we identified the regulatory effects of these IFNs on IL-8 mRNA expression and IL-8 production by hum...

Human normal, periodontitis and overgrown gingival fibroblasts were assayed concerning proinflammatory cytokines secreting ability. Stimulating effect of gingival overgrowth-inducing drugs such as phenytoin, nifedipine or cyclosporine A and LPS on the secretion ability of each fibroblast specimen was also estimated. The supernatant of cultured medium was assayed cytokines by ELISA techniques. I...

Rikkosan is a traditional Kampo medicine using the control of oral pain caused by dental caries, pulpitis, periodontitis and stomatitis. In order to provide evidence for its clinical effects, we herein investigated whether Rikkosan inhibits the production of pro-inflammatory substances in human and mouse models of inflammation. Rikkosan alone did not induce prostaglandin E2 (PGE2) production, b...

IL-1β contributes to connective tissue destruction in part by up-regulating stromelysin-1 (MMP-3), which in fibroblasts is a focal adhesion-dependent process. Protein tyrosine phosphatase-α (PTPα) is enriched in and regulates the formation of focal adhesions, but the role of PTPα in connective tissue destruction is not defined. We first examined destruction of periodontal connective tissues in ...