The dermal extracellular matrix (ECM) provides strength and resiliency to skin. The ECM consists mostly of type I collagen fibrils, which are produced by fibroblasts. Binding of fibroblasts to collagen fibrils generates mechanical forces, which regulate cellular morphology and function. With aging, collagen fragmentation reduces fibroblast-ECM binding and mechanical forces, resulting in fibrobl...

Recently we reported that human dermal fibroblasts, or conditioned media obtained from such cells, affect the growth of human melanoma cells as a direct function of tumor progression: melanoma cells obtained from early-stage (metastatically incompetent) primary lesions were growth inhibited, whereas cells obtained from more advanced (metastatically competent) primary lesions, or metastases, wer...

Culture technology have permitted the generation of an artificial skin using human neonatal stem cells. A major advantage of this model is that epithelial-mesenchymal interactions are maintained. We have studied some morphological aspects concerning tissue organisation and cell differentiation using immunohistochemistry and electron microscopy. The epidermal equivalent was composed by a stratif...

Chemotherapy is one of the main strategies for reducing the rate of cancer progression or, in some cases, curing the tumour. Since a great number of chemotherapeutic agents are cytotoxic compounds, i. e. similarly affect normal and neoplastic cells, application of antitumour drugs is preferred in cancer management and therapy. In this study, the cytotoxicity of diversin was evaluated in 5637 ce...

Dermal fibroblasts are required for structural integrity of the skin and for hair follicle development. Uniform Wnt signaling activity is present in dermal fibroblast precursors preceding hair follicle initiation, but the functional requirement of dermal Wnt signaling at early stages of skin differentiation and patterning remains largely uncharacterized. We show in mice that epidermal Wnt ligan...

plant-based drugs are regarded promising recently. scrophularia has been shown various biological activities such as antimicrobial, antitumor and anti-inflammatory properties. according to pervious researches, bacteria are becoming resistant to some kinds of antibiotics, so it is prominent to find more reliable sources against them. here staphylococcus aureus as a common type of gram- positive ...

Skin photodamage, which is induced by ultraviolet (UV) radiation, a prevalent cause of skin damage. In this study, transdermal drug delivery system developed for the topical treatment composed tetrahedral framework nucleic acids (tFNAs) and lipoic acid (LA). The tFNAs-LA (TLA) nanocomposite exhibits excellent biocompatibility, as well antioxidant, anti-apoptotic, anti-inflammatory capabilities....

Adipose‑derived stem cells (ADSCs) are known to secrete various cytokines, which affect fibroblast function through paracrine effects. In the present study, the paracrine effects of ADSCs on the function and senescence of young and aged human dermal fibroblasts (HDFs) were investigated in vitro. ADSCs and HDFs were isolated from healthy donors and flow cytometry was used for immunophenotype ide...

Diabetic foot ulcerations could result in serious consequences such as amputations. The up-regulation of matrix metalloproteinases and down-regulation of TIMP1 were remarked as distinctive biological characteristics in the diabetic dermal fibroblast. The current study was performed in order to clarify the effect of high glucose on formation of diabetic dermal fibroblast cell. In addition, the e...

OBJECTIVE To determine whether acellular human dermis is degraded by matrix metalloproteinases (MMPs), a large class of matrix-degrading enzymes. METHODS The degradation of acellular human dermis specimens was evaluated in vitro. Wild-type murine fibroblasts with a broad-spectrum MMP inhibitor, GM6001, and MMP-2-deficient fibroblasts were placed on the basement membrane and dermal surfaces of...