Tetracaine (N,N-dimethylaminoethyl-4-butylaminobenzoate) and related N,N-dialkylaminoethyl substituted benzoic acid esters have been used to characterize the high-affinity binding site for aromatic amine noncompetitive antagonists in the Torpedo nicotinic acetylcholine receptor (nAChR). [H]Tetracaine binds at equilibrium to a single site with a Keq value of 0.5 mM in the absence of agonist or p...

Mutations in the human LMNA gene underlie many laminopathic diseases, including EmeryDreifuss muscular dystrophy (EDMD); however, a mechanistic link between the effect of mutations on lamin filament assembly and disease phenotypes has not been established. We studied the ∆K46 C. elegans lamin mutant, corresponding to EDMD-linked ∆K32 in human lamins A/C. Cryo-electron tomography of lamin ∆K46 f...

receptor from Torpedo electric organ with channel blockers (Giraudat et al., 1986; Hucho et al., 1986; Pedersen and Cohen, 1990; White and Cohen, 1992), together with mutagenesis of the affinity-labeled amino acids or their homologs in muscle-type 2a1b1gd receptor Pierre-Jean Corringer,* Sonia Bertrand,† Jean-Luc Galzi,*# Anne Devillers-Thiéry,* Jean-Pierre Changeux,*‡ and Daniel Bertrand† *Neu...

Several subfamilies of the salmonid Hpa I short interspersed element (SINE) family were isolated from salmonid genomes and were sequenced. For each genomic locus that represented the subfamily, amplification by PCR of the orthologous loci in the 12 fish aflowed us to determine the order of branching of the Pacific salmonid species. The deduced phylogeny suggests three evolutionary lines, namely...

108$ 3 Johns A, Lategan TW Lodge NJ, Ryan US, van Breemen C, Adams DJ. Calcium entry through receptor-operated channels in bovine pulmonary artery endothelial cells. Tissue Cell 1987;19:1-13 4 Ryan US, Avdonin P\( Pozin EYa, Popov EG, Danilov SM, Tkachuk VA. Influence of vasoactive agents on cytoplasmic free calcium concentration in INDO-1 loaded vascular endothelial cells. J App! Physiol (in p...

Trans-urocanic acid (trans-UCA) is synthesized in the skin, liver, and brain. It a major natural moisturizing factor skin maintains its pH. In it isomerizes to cis-UCA following exposure UVR. Both isomers fulfill multiple roles health disease. Cis-UCA has immunomodulatory properties linked with several cutaneous diseases such as cancer, atopic dermatitis, urticaria associates systemic including...