Horseradish peroxidase (HRP) catalyzes the oxidative para-dechlorination of the environmental pollutant/carcinogen 2,4,6-trichlorophenol (2,4,6-TCP). A possible mechanism for this reaction is a direct oxygen atom transfer from HRP compound I (HRP I) to trichlorophenol to generate 2,6-dichloro 1,4-benzoquinone, a two-electron transfer process. An alternative mechanism involves two consecutive on...

For the exploration of the intrinsic reactivity of two key active species in the catalytic cycle of horseradish peroxidase (HRP), Compound I (HRP-I) and Compound II (HRP-II), we generated in situ [Fe(IV) O(TMP(+.) )(2-MeIm)](+) and [Fe(IV) O(TMP)(2-MeIm)](0) (TMP=5,10,15,20-tetramesitylporphyrin; 2-MeIm=2-methylimidazole) as biomimetics for HRP-I and HRP-II, respectively. Their catalytic acti...

This paper presents a radio-frequency (RF) antenna as sensor to detect Horseradish peroxidase (HRP). At the core of proposed approach is graphene film deposited on stub connected an RF antenna. The doctor bladed stub. then properly chemically functionalized in order presence We validate proof-of-concept operation HRP concentration detection by measuring frequency shift reflection coefficient us...

Oxidation of 1,3,4,5-tetrahydro-2H-1,5-benzodiazepine oximes catalysed by horseradish peroxidase (HRP) and recombinant Coprinus cinereus (rCiP) was studied spectrophotometrically. The reaction rate dependences on the substrate hydrogen peroxide concentrations were investigated; values apparent KM Vmax, catalytic, oxidation reduction constants (kcat, kox kred, respectively) calculated. reactivit...

We have developed a humanoid robot HRP-1S that is capable of simultaneous whole body motion control. In phase one of the Humanoid Robotics Project (HRP) of the Ministry of Economy, Trade and Industry of Japan, Honda R&D Co. Ltd has produced humanoid robot HRP-1 as a humanoid research platform to ease the development of good applications for humanoid robots in phase two of HRP. However, HRP-1 co...

The uptake, distribution, and fate of particulate horseradish peroxidase (HRP)-anti HRP aggregates has been studied in homogeneous monolayers of mouse macrophages in vitro. Macrophages rapidly interiorize the immune complexes after binding to the cell surface. The rate of interiorization is maximal for complexes formed in a broad zone of 4-fold antibody excess to equivalence and corresponds to ...

Human serum albumin (HSA) is an intrinsic protein and important carrier that transports endogenous as well as exogenous substances across cell membranes. Herein, we have designed and prepared resveratrol (RV)-loaded HSA nanoparticles conjugating RGD (arginine-glycine-aspartate) via a polyethylene glycol (PEG) "bridge" (HRP-RGD NPs) for highly effective targeted pancreatic tumor therapy. HRP-RGD...

Microfluidic technology has now become a novel sensing platform where different analytical steps, biological recognition materials and suitable transducers can be cleverly integrated yielding a new sensor generation. A novel "lab-on-a-chip" strategy integrating an electrokinetic magnetic bead-based electrochemical immunoassay on a microfluidic chip for reliable control of permitted levels of ze...

Immobilization of single antioxidant enzyme systems was frequently studied in the past, however, there is a lack reliable reports on co-immobilization such enzymes. Here, an cascade involving superoxide dismutase (SOD) and horseradish peroxidase (HRP) successfully immobilized titania nanosheets (TNS) by sequential adsorption method using poly(diallyldimethylammonium chloride) (PDADMAC) poly(sty...

Hepatoma-derived growth factor-related proteins (HRP) comprise a family of 6 members, which the biological functions are still largely unclear. Here we show that during embryogenesis HRP-3 is strongly expressed in the developing nervous system. At early stages of development HRP-3 is located in the cytoplasm and neurites of cortical neurons. Upon maturation HRP-3 relocalizes continuously to the...