Pistachio hull contains anacardic acids. It is a salicylic acid with side chain either saturated or unsaturated. This work focused on the efficient extraction of acids using solvent. Six aliquots were weighed and extracted hexane, ethyl acetate, mixture H2O/MeOH/HCl. Hexane was most solvent due to its ability dissolve Only one standard used quantify all them since does not affect wavelength gre...

Quantification of the lipid content in liposomal adjuvants for subunit vaccine formulation is of extreme importance, since this concentration impacts both efficacy and stability. In this paper, we outline a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method that allows for the rapid and simultaneous quantification of lipid concentrations within lipos...

A simple, accurate and sensitive high-performance liquid chromatography (HPLC) method was developed, validated and applied to the determination of either theophylline or paracetamol in milk-based samples. The method allowed drug quantification in fresh and powdered milk with a relatively short run time of analysis and it was also successfully applied to the quantification of the drugs in solid ...

Conventional methods for extracting and quantifying phenolic compounds in citrus rinds are time consuming. Rapid methods for extracting and quantifying phenolic compounds were developed by comparing three extraction solvent combinations (80:20 v/v ethanol:H2O; 70:29.5:0.5 v/v/v methanol:H2O:HCl; and 50:50 v/v dimethyl sulfoxide (DMSO):methanol) for effectiveness. Freeze-dried, rind powder was e...

The qualitative and quantitative analysis of active constituents in Fructus Psoraleae is presented by high-performance liquid chromatography (HPLC) coupled with different detections. Extracts of Fructus Psoraleae were examined by HPLC with ion trap mass spectrometry (IT-MS) and 18 major compounds of coumarins, benzofuran glycosides, flavonoids, and meroterpene were identified. The determination...

The potency of influenza vaccine is determined based on its hemagglutinin (HA) content. In general, single radial immunodiffusion (SRID) assay has been utilized as the standard method to measure HA content. However, preparation of reagents for SRID such as antigen and antibody takes approximately 2~3 months, which causes delays in the development of influenza vaccine. Therefore, quantification ...

The preliminary research work carried to find out the concentration of Gentamicin in the raw material. Two different techniques used in this study for the quantification of Gentamicin. They are microbiological assay method and reversed phase HPLC. The concentration of Gentamicin was quantified even at very lower concentration by microbiological assay method and it is sensitive. The test organis...

Caffeine has traditionally been used to assess and control the metrological characteristics of liquid chromatographs. In recent years, with increasing requirements for traceability measurement results, use caffeine as a reagent verification, calibration, type approval tests started contradict effective regulations governing uniformity. This study aims develop test procedure quantification in vi...