The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, which measures the reduction of MTT, is commonly used to validate the viability of metabolically active cells. This study was conducted to evaluate and validate the MTT assay to assess the spermatozoal viability of Nili-Ravi buffalo bulls and compare the efficiency of the test with the supravital staining technique (e...

Tanacetum parthenium (feverfew) is an herb that is commercialized worldwide as a therapeutic treatment for migraine. Its pharmacological effect is mainly due to the presence of the sesquiterpene lactone parthenolide as well as of flavonoids. So far, there are no studies on standardization of pre-formulations or phytomedicines containing this herb. The present study aimed at developing a pre-for...

BACKGROUND/AIMS Erythrocytes may enter eryptosis, a suicidal death characterized by cell shrinkage and phosphatidylserine exposure at the erythrocyte outer membrane. Susceptibility to eryptosis is enhanced in aged erythrocytes and stimulated by NFκB-inhibitors Bay 11-7082 and parthenolide. Here we explored whether expression of NFκB and susceptibility to inhibitor-induced eryptosis is sensitive...

OBJECTIVE This study evaluated in vitro cell viability by the colorimetric MTT stands for 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay compared to image analysis by CellProfiler® software. MATERIALS AND METHODS Hepatoma (Hepa-1c1c7) and fibrobla...

Allium hirtifolim (Persian Shallot) belongs to Allium genus (Alliaceae family). We investigated the in vitro effects of chloroformic extract of A. hirtifolium and its Allicin on the proliferation of HeLa (cervical cancer), MCF7 (human, caucasion, breast, adenocarcinoma) and L929 (mouse, C3H/An, connective) cell lines. Our results showed that components of A. hirtifolium might inhibit proliferat...

The sesquiterpene lactone parthenolide has recently attracted considerable attention owing to its promising antitumor properties, in particular in the context of stem-cell cancers including leukemia. Yet, the lack of viable synthetic routes for re-elaborating this complex natural product has represented a fundamental obstacle toward further optimization of its pharmacological properties. Here, ...

Cancer stem cells play a central role in the pathogenesis of nasopharyngeal carcinoma and contribute to both disease initiation and relapse. In this study, cyclooxygenase-2 (COX-2) was found to regulate cancer stem-like side population cells of nasopharyngeal carcinoma cells and enhance cancer stem-like cells' characteristics such as higher colony formation efficiency and overexpression of stem...

Plants continue to play a critical role in the discovery of effective compounds, especially anticancer drugs. Abutilon pannosum has been historically used as therapeutic plant medicinal system. In this study, an ethanolic crude extract was prepared from dried powder A. and subsequently fractionated produce chloroform, butanol, water fractions. The fractions were tested for their cytotoxic actio...

Abstract Punica granatum peel is a significant source of polyphenolic compounds that have potential uses as drugs. This study aimed to obtain and purify derived from pomegranate evaluate their anticancer activity in cell lines. Polyphenols the were extracted using hybrid ultrasound‐microwave technology. Different mass:volume ratios water:ethanol used during extraction. Purification was performe...

Institute of Advanced Chemistry of Cata E-08034 Barcelona, Spain. E-mail: recgma@ Networking Centre on Bioengineering, Biom Spain University of Barcelona, Department of Chemistry, Mart́ı i Franquès 1-11, E-08028 † Electronic supplementary information (E H-NMR and C-NMR spectra, HPLC pro thermal denaturation experiments, nati conjugates, MTT colorimetric assay, tr conditions, ow cytometry analys...