REASONS FOR PERFORMING STUDY African horse sickness is an insect-transmitted, noncontagious disease of equids caused by African horse sickness virus (AHSV). Mortality can exceed 90% in fully susceptible horse populations. A live-attenuated (modified live) cell-culture-adapted (MLV) polyvalent AHSV vaccine is widely used to control African horse sickness in endemic areas in southern Africa. Fiel...

Native and reversed horse serum albumin are indistinguishable when tested immunologically by means of the precipitin reaction.

The method of cultivation most spirochaetes is not yet generally available to every bacteriological laboratory; associated with considerable, sometimes even insurmountable difficulties, both in relations preparation nutrient media and technique itself, especially terms obtaining pure cultures. For one usually needs blood serum human, horse, rabbit, ram or guinea pig, ascitic fluid hydrocele flu...

The most used and reliable indicator of Equine infectious anemia virus (EIAV) infection is the detection of its specific antibodies in horse serum. In the present study, the performance of two commercial ELISA tests for the detection of EIAV antibodies as well as the potential advantages of their use as an EIAV infection screening tool were evaluated in 302 horse serum samples. Both ELISA assay...

Using immunoradiometric assays (IRMAs) from Hybritech Inc. (H) and Nichols Institute Diagnostics (ND), we measured somatotropin (human growth hormone, hGH) in serum samples obtained every 20 min for 24 h from 10 prepubertal subjects with short stature. Results obtained with the ND reagents were 2.74 times greater than those obtained with the H reagents (P = 0.00001, r = 0.94, SEE = 3.9, n = 720...

Chicken egg yolk broth medium containing 10% (vol/vol) phosphate-buffered saline extract of egg yolk instead of horse serum showed better growth-promoting activity for Mycoplasma pneumoniae than did Chanock medium and was effective in promoting the growth of small numbers of M. pneumoniae. Moreover, the phosphate-buffered saline extract of egg yolk proved superior to horse serum in the followin...

1. The method of Kekwick & Mackay (1954) for the fractionation of human plasma protems was used successfully for the separation of the serum proteins ofguinea pig, rat, rabbit, cat, dog, monkey, ox, horse and sheep. 2. By variations in pH, ionic strength, temperature and solvent concentration, three fractions consisting essentially of a mixture of xand #globulins (G2); y-globulin (G 3) and albu...

Blood samples were collected from 20 healthy miniature Caspian horses at 37 °C. Isolation of cryoglobulin was performed based on a standard method in present study. Harvested sera were kept at 4 °C for two hours and then examined for cryoglubolin. Four serum samples containing precipitate Suspicious of containing cryoglobulin were selected. Subsequently serum protein electrophoresis was perform...

A 61-year-old woman visited our clinic to continue rabies post-exposure vaccination. She had sustained a dog bite on the right calf during a journey to South Asia seven days earlier. Immediately after the bite she received several injections on both upper arms, as well as around the bite site, at a local clinic. Active immunisation with Verorab 1 , intradermally applied at both deltoid areas on...

The antibiotic activity of cephalothin, cephaloridine, cephalexin, cephaloglycin, cefazolin, and cefamandole was determined after storage for up to 30 days in horse serum at -10 and 4 degrees C. Cephalothin, cefamandole, cefazolin, and cephalexin were stable for at least 30 days at -10 degrees C, whereas cephaloridine lost 29% of its initial activity and cephaloglycin lost more than 50%. Cefama...